Interdisciplinary Research Center in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Lahore, 54000, Pakistan.
Interdisciplinary Research Center in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, Lahore, 54000, Pakistan.
Int J Biol Macromol. 2018 Dec;120(Pt A):721-728. doi: 10.1016/j.ijbiomac.2018.08.103. Epub 2018 Aug 24.
Nanoparticles are well recognized for their biological applications including tissue-regeneration due to large surface area and chemical properties. In this study, K-doped zinc oxide (ZnO) nanoparticles containing porous hydrogels were synthesized via freeze gelation. The morphology and pore dimensions were studied by scanning electron microscopy (SEM). The chemical structural analysis of the synthesized hydrogels was investigated by Fourier Transform Infrared (FTIR) spectroscopy. In swelling studies, material containing ZnO nanoparticles with 2% potassium dopant concentration CLH-K) showed greater degree of swelling as compared to all other materials. The degradation studied was tested in three different degradation media, i.e. phosphate buffer saline (PBS), lysozyme and hydrogen peroxide and relatively higher degradation was seen in hydrogen peroxide. The synthesized hydrogels were implanted on the chick chorioallantoic membrane (CAM) to investigate their angiogenic potential. The CLH-K hydrogel stimulated angiogenesis greater than all other materials; blood vessels were attached and grown inside this scaffold, showing its strong angiogenic potential.
纳米粒子因其大表面积和化学性质而在生物应用中得到广泛认可，包括组织再生。在这项研究中，通过冷冻凝胶化合成了含有多孔水凝胶的 K 掺杂氧化锌（ZnO）纳米粒子。通过扫描电子显微镜（SEM）研究了形态和孔径尺寸。通过傅里叶变换红外（FTIR）光谱研究了合成水凝胶的化学结构分析。在溶胀研究中，与所有其他材料相比，含有 2%钾掺杂浓度 ZnO 纳米粒子的材料（CLH-K）显示出更大的溶胀程度。在三种不同的降解介质，即磷酸盐缓冲盐水（PBS）、溶菌酶和过氧化氢中进行的降解研究中，在过氧化氢中观察到相对较高的降解。将合成水凝胶植入鸡胚绒毛尿囊膜（CAM）上，以研究其血管生成潜力。CLH-K 水凝胶刺激血管生成的能力大于所有其他材料；血管附着并在这个支架内生长，显示出其强大的血管生成潜力。
