Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China.
Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China; College of Agriculture, Ludong University, Yantai, Shandong, 264025, China.
Plant Physiol Biochem. 2019 Sep;142:351-362. doi: 10.1016/j.plaphy.2019.07.025. Epub 2019 Jul 31.
Cassava is an important tropical crop with strong resistance to drought stress. The chloroplast, the site of photosynthesis, is sensitive to stress, and the drought-response proteins in cassava chloroplasts are worthy of investigation. In this study, cassava leaves were collected for ultra-structure observation from plants subjected to different drought stress conditions. Our results showed that drought stress can promote starch accumulation in cassava chloroplasts. To evaluate changes in chloroplast proteins under different drought conditions, two-dimensional electrophoresis was performed using purified chloroplasts, which resulted in the identification of 26 unique chloroplast proteins responsive to drought stress. These drought-responsive proteins are predominantly related to photosynthesis, carbon and nitrogen metabolism, and amino acid metabolism. Among them, most photosynthesis-related proteins are downregulated, with decreases in photosynthetic parameters upon drought stress. Several proteins associated with carbon and nitrogen metabolism, including rubisco and carbonic anhydrase, were upregulated, which might promote drought tolerance in cassava by enhancing the carbohydrate conversion efficiency and protecting the plant from oxidative stress. Our proteomic data not only provide insight into the complement of proteins in cassava chloroplasts but also further our overall understanding of drought-responsive proteins in cassava chloroplasts.
木薯是一种重要的热带作物，具有很强的抗旱能力。叶绿体是光合作用的场所，对胁迫敏感，木薯叶绿体中的干旱响应蛋白值得研究。本研究收集了不同干旱胁迫条件下的木薯叶片进行超微结构观察。结果表明，干旱胁迫可促进木薯叶绿体中淀粉的积累。为了评估不同干旱条件下叶绿体蛋白的变化，用纯化的叶绿体进行了二维电泳，鉴定出 26 种对干旱胁迫有响应的独特叶绿体蛋白。这些干旱响应蛋白主要与光合作用、碳氮代谢和氨基酸代谢有关。其中，大多数与光合作用相关的蛋白下调，干旱胁迫下光合作用参数下降。一些与碳氮代谢相关的蛋白，包括 Rubisco 和碳酸酐酶，上调，可能通过提高碳水化合物转化效率和保护植物免受氧化应激来增强木薯的耐旱性。本蛋白质组学数据不仅提供了对木薯叶绿体中蛋白质组成的深入了解，也进一步加深了我们对木薯叶绿体中干旱响应蛋白的整体认识。
