Zhou Ying, Li Huiying, Chen Haoran, Yang Xiaoqin, Yu Tingting, Wang Yushuang, Wang Yujue, Jiang Keting, Wang Yan, Chen Zhanyu, Cui Xiyan
College of Life Sciences, Jilin Agricultural University, Changchun 130118, China.
Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, China.
Plants (Basel). 2022 Apr 26;11(9):1173. doi: 10.3390/plants11091173.
Roots are generally the critical drought sensors, but little is known about their molecular response to drought stress. We used the drought-tolerant soybean variety 'Jiyu 47' to investigate the differentially expressed proteins (DEPs) in soybean roots during the seedling stage based on the tandem mass tag (TMT) proteomics analysis. Various expression patterns were observed in a total of six physiological parameters. A total of 468 DEPs (144 up-regulated and 324 down-regulated) among a total of 8687 proteins were identified in response to drought stress in 24 h. The expression of DEPs was further validated based on quantitative real-time PCR of a total of five genes (i.e., , , k , and ) involved in the glutathione biosynthesis. Results of enrichment analyses revealed a coordinated expression pattern of proteins involved in various cellular metabolisms responding to drought stress in soybean roots. Our results showed that drought stress caused significant alterations in the expression of proteins involved in several metabolic pathways in soybean roots, including carbohydrate metabolism, metabolism of the osmotic regulation substances, and antioxidant defense system (i.e., the glutathione metabolism). Increased production of reduced glutathione (GSH) enhanced the prevention of the damage caused by reactive oxygen species and the tolerance of the abiotic stress. The glutathione metabolism played a key role in modifying the antioxidant defense system in response to drought stress in soybean roots. Our proteomic study suggested that the soybean plants responded to drought stress by coordinating their protein expression during the vegetative stage, providing novel insights into the molecular mechanisms regulating the response to abiotic stress in plants.
根系通常是关键的干旱感受器，但对于它们对干旱胁迫的分子响应却知之甚少。我们利用耐旱大豆品种‘吉育47’，基于串联质谱标签（TMT）蛋白质组学分析，研究了大豆幼苗期根系中差异表达蛋白（DEPs）。在总共六个生理参数中观察到了各种表达模式。在24小时内，共鉴定出8687种蛋白质中的468种差异表达蛋白（144种上调和324种下调）以响应干旱胁迫。基于对总共五个参与谷胱甘肽生物合成的基因（即， ， k ，和 ）的定量实时PCR，进一步验证了差异表达蛋白的表达。富集分析结果揭示了大豆根系中参与各种细胞代谢以响应干旱胁迫的蛋白质的协同表达模式。我们的结果表明，干旱胁迫导致大豆根系中参与多种代谢途径的蛋白质表达发生显著变化，包括碳水化合物代谢、渗透调节物质代谢和抗氧化防御系统（即谷胱甘肽代谢）。还原型谷胱甘肽（GSH）产量的增加增强了对活性氧造成的损伤的预防以及对非生物胁迫的耐受性。谷胱甘肽代谢在响应大豆根系干旱胁迫时修饰抗氧化防御系统中起关键作用。我们的蛋白质组学研究表明，大豆植株在营养生长阶段通过协调其蛋白质表达来响应干旱胁迫，为调控植物对非生物胁迫响应的分子机制提供了新的见解。
