Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
Key Laboratory of Biology and Genetic Resources of Torpical Crops, Ministry of Agriculture, Haikou, 571101, China.
BMC Plant Biol. 2018 Dec 4;18(1):329. doi: 10.1186/s12870-018-1528-6.
CC-type glutaredoxins (GRXs) are plant-specific glutaredoxin, play regulatory roles in response of biotic and abiotic stress. However, it is not clear whether the CC-type GRXs are involve in drought response in cassava (Manihot esculenta), an important tropical tuber root crop.
Herein, genome-wide analysis identified 18 CC-type GRXs in the cassava genome, of which six (namely MeGRXC3, C4, C7, C14, C15, and C18) were induced by drought stress in leaves of two cassava cultivars Argentina 7 (Arg7) and South China 124 (SC124). Exogenous abscisic acid (ABA) application induced the expression of all the six CC-type GRXs in leaves of both Arg7 and SC124 plants. Overexpression of MeGRXC15 in Arabidopsis (Col-0) increases tolerance of ABA on the sealed agar plates, but results in drought hypersensitivity in soil-grown plants. The results of microarray assays show that MeGRXC15 overexpression affected the expression of a set of transcription factors which involve in stress response, ABA, and JA/ET signalling pathway. The results of protein interaction analysis show that MeGRXC15 can interact with TGA5 from Arabidopsis and MeTGA074 from cassava.
CC-type glutaredoxins play regulatory roles in cassava response to drought possibly through ABA signalling pathway.
CC 型谷氧还蛋白（GRXs）是植物特有的谷氧还蛋白，在生物和非生物胁迫的响应中发挥调节作用。然而，目前尚不清楚 CC 型 GRX 是否参与木薯（Manihot esculenta）对干旱的响应，木薯是一种重要的热带块根作物。
本文通过全基因组分析，在木薯基因组中鉴定出 18 个 CC 型 GRX，其中 6 个（即 MeGRXC3、C4、C7、C14、C15 和 C18）在两个木薯品种阿根廷 7（Arg7）和华南 124（SC124）叶片中受干旱胁迫诱导。外源脱落酸（ABA）处理诱导 Arg7 和 SC124 植株叶片中所有 6 个 CC 型 GRX 的表达。在拟南芥（Col-0）中过表达 MeGRXC15 增加了对 ABA 密封琼脂平板的耐受性，但在土壤生长的植物中导致干旱敏感性增加。微阵列分析的结果表明，MeGRXC15 过表达影响了一组参与应激反应、ABA 和 JA/ET 信号通路的转录因子的表达。蛋白质相互作用分析的结果表明，MeGRXC15 可以与拟南芥的 TGA5 和木薯的 MeTGA074 相互作用。
CC 型谷氧还蛋白在木薯响应干旱过程中可能通过 ABA 信号通路发挥调节作用。
