使用骨髓间充质干细胞和碳酸羟基磷灰石载体在犬人工颌骨裂隙模型中的骨再生
Bone Regeneration in a Canine Model of Artificial Jaw Cleft Using Bone Marrow-Derived Mesenchymal Stem Cells and Carbonate Hydroxyapatite Carrier.
作者信息
Abe Takaharu, Sumi Keisuke, Kunimatsu Ryo, Oki Nanae, Tsuka Yuji, Awada Tetsuya, Nakajima Kengo, Sugiyama Masaru, Tanimoto Kotaro
机构信息
Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima, Japan.
Department of Public Oral Health, Hiroshima University Graduate School of Biomedical & Health Sciences, Hiroshima Japan.
出版信息
Cleft Palate Craniofac J. 2020 Feb;57(2):208-217. doi: 10.1177/1055665619868868. Epub 2019 Aug 18.
OBJECTIVE
Cleft lip and palate (CLP) is a common anomaly of the orofacial region. Mesenchymal stem cell (MSC) transplantation has been a focus of regenerative medicine, and its application to the repair of bone defects in patients with CLP is highly anticipated. This study investigated the potential for using MSCs to regenerate bone in a jaw cleft as well as the survival of transplanted MSCs using a canine model of CLP.
DESIGN
Mesenchymal stem cells collected from the bone marrow of beagle dogs were transplanted along with carbonate hydroxyapatite into jaw clefts in beagle dogs. Mesenchymal stem cells labeled with fluorescent silica nanoparticles were also transplanted, and a histological analysis was performed 3 months later to evaluate MSC survival.
RESULTS
Carbonate hydroxyapatite regeneration into bone was enhanced by cotransplantation of MSCs. The survival rate of MSCs transplanted after 3 months was 5.7%.
CONCLUSIONS
Transplanted MSCs promote bone regeneration, although their survival rate is low.
目的
唇腭裂（CLP）是口腔面部区域常见的先天性畸形。间充质干细胞（MSC）移植一直是再生医学的研究热点，其在CLP患者骨缺损修复中的应用备受期待。本研究利用犬CLP模型，探讨了MSC在颌裂骨再生中的潜力以及移植MSC的存活情况。
设计
从比格犬骨髓中收集的间充质干细胞与碳酸羟基磷灰石一起移植到比格犬的颌裂中。还移植了用荧光二氧化硅纳米颗粒标记的间充质干细胞，并在3个月后进行组织学分析以评估MSC的存活情况。
结果
MSC共移植可增强碳酸羟基磷灰石向骨的再生。3个月后移植的MSC存活率为5.7%。
结论
尽管移植的MSC存活率较低，但可促进骨再生。
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