Korte K, Casey M L
J Chromatogr. 1982 Oct 8;232(1):47-53. doi: 10.1016/s0378-4347(00)86006-5.
A simple and rapid method for separation of six major phospholipids and four major neutral lipids from cell extracts by one-dimensional preadsorbent thin-layer chromatography was developed. Due to the inert characteristics of the preadsorbent layer (Celite) no separation occurs until the sample reaches the preadsorbent (Celite)-silica gel junction. The compounds were applied to the preadsorbent (Celite) area (625 mm2) in 10-microliters aliquots (total volume of 0.15 ml). By this method, samples can be applied rapidly in large volumes without respotting any area of the preadsorbent layer. The time required to apply one sample was reduced considerably (2 min) compared to conventional methods (10 min). Since all the compounds move with the solvent front as a sharp, narrow band to the preadsorbent (Celite)-silica gel boundary, excellent separation was achieved when up to 650 micrograms of lipid material was applied on each lane (25 mm wide). Thus, this method is suitable for the separation of relatively large amounts of radiolabeled and non-radiolabeled lipids and free fatty acids from extracts of biological fluids, tissues, or cells maintained in monolayer culture.
开发了一种通过一维预吸附剂薄层色谱从细胞提取物中分离六种主要磷脂和四种主要中性脂质的简单快速方法。由于预吸附剂层（硅藻土）的惰性特性，在样品到达预吸附剂（硅藻土）-硅胶交界处之前不会发生分离。将化合物以10微升等分试样（总体积0.15毫升）施加到预吸附剂（硅藻土）区域（625平方毫米）。通过这种方法，可以快速大量地施加样品，而无需重新点样预吸附剂层的任何区域。与传统方法（10分钟）相比，施加一个样品所需的时间大大减少（2分钟）。由于所有化合物都作为一个尖锐、狭窄的带随溶剂前沿移动到预吸附剂（硅藻土）-硅胶边界，当每条泳道（25毫米宽）上施加高达650微克的脂质材料时，实现了出色的分离。因此，该方法适用于从生物流体、组织或单层培养的细胞提取物中分离相对大量的放射性标记和非放射性标记的脂质以及游离脂肪酸。
