外周血神经元衍生外泌体中 Aβ42、Tau 和 P-T181-tau 的评估与脑脊液之间的一致性。
Concordance between the assessment of Aβ42, T-tau, and P-T181-tau in peripheral blood neuronal-derived exosomes and cerebrospinal fluid.
机构信息
Innovation Center for Neurological Disorders, Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, P.R.China.
Department of Neurology, the Affiliated Hospital of Guizhou Medical University, Guizhou, P.R.China.
出版信息
Alzheimers Dement. 2019 Aug;15(8):1071-1080. doi: 10.1016/j.jalz.2019.05.002.
INTRODUCTION
Neuronal-derived exosomal Aβ42, T-tau, and P-T181-tau have been demonstrated to be biomarkers of Alzheimer's disease (AD). However, no study has assessed the association of Aβ42, T-tau, and P-T181-tau between exosomes and CSF.
METHODS
This was a multicenter study with two-stage design. The subjects included 28 AD patients, 25 aMCI patients, and 29 controls in the discovery stage; the results of which were confirmed in the validation stage (73 AD, 71 aMCI, and 72 controls).
RESULTS
The exosomal concentrations of Aβ42, T-tau, and P-T181-tau in AD group were higher than those in aMCI and control groups (all P < .001). The level of each exosomal biomarker was highly correlated with that in CSF.
DISCUSSION
This study verified the agreement between CSF and blood exosomal biomarkers and confirmed that exosomal Aβ42, T-tau, and P-T181-tau have the same capacity as those in CSF for the diagnosis of AD and aMCI.
简介
已证实神经元衍生的外泌体 Aβ42、T- tau 和 P-T181-tau 是阿尔茨海默病（AD）的生物标志物。然而，尚无研究评估 AD 患者脑脊液（CSF）与外泌体中 Aβ42、T- tau 和 P-T181-tau 的关联。
方法
这是一项多中心、两阶段设计的研究。在发现阶段纳入 28 例 AD 患者、25 例 aMCI 患者和 29 例对照者；在验证阶段纳入 73 例 AD 患者、71 例 aMCI 患者和 72 例对照者。
结果
AD 组外泌体 Aβ42、T- tau 和 P-T181-tau 的浓度高于 aMCI 组和对照组（均 P<.001）。每个外泌体生物标志物的水平与 CSF 中该标志物的水平高度相关。
讨论
本研究验证了 CSF 和血液外泌体生物标志物之间的一致性，并证实外泌体 Aβ42、T- tau 和 P-T181-tau 与 CSF 一样，可用于 AD 和 aMCI 的诊断。
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