Rikihisa Y, Jiang B M
Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Antimicrob Agents Chemother. 1988 Jul;32(7):986-91. doi: 10.1128/AAC.32.7.986.
Inhibition of the proliferation of Ehrlichia risticii cultured in murine macrophage P388D1 cells by eight antibiotics was evaluated by indirect fluorescent-antibody staining with an antiserum specific to E. risticii. There was a negative correlation between the percentage of infected cells and the log10 of the concentrations of all antibiotics examined. The ranks of the antibiotics in the order of 50% inhibitory concentrations (on a microgram-per-milliliter basis) after 48 h of exposure were as follows: demeclocycline, doxycycline, and oxytetracycline less than minocycline less than rifampin less than tetracycline less than erythromycin and nalidixic acid. When the antibiotics were removed after 48 h of incubation, continuous inhibition of proliferation was evident at 72 h. At 96 h regrowth of the organisms occurred in most of the cultures. The rate of regrowth was the highest with nalidixic acid, followed by erythromycin, at all concentrations of the antibiotic tested. Regrowth was observed with less than 0.1 microgram of minocycline per ml and less than 0.01 microgram of oxytetracycline, tetracycline, and doxycycline per ml. With more than 0.01 microgram of demeclocycline per ml, however, the inhibition persisted for up to 72 h after removal of the antibiotic. These results indicate that demeclocycline was slightly more effective than doxycycline, oxytetracycline, and minocycline in eliminating E. risticii in macrophages in vitro, whereas tetracycline and rifampin were less effective. Nalidixic acid and erythromycin were ineffective.
采用针对瑞氏埃里希体的抗血清进行间接荧光抗体染色，评估了8种抗生素对在鼠巨噬细胞P388D1中培养的瑞氏埃里希体增殖的抑制作用。感染细胞的百分比与所有检测抗生素浓度的log10之间呈负相关。暴露48小时后，按50%抑制浓度（以微克/毫升为单位）排序的抗生素顺序如下：去甲金霉素、强力霉素和土霉素小于米诺环素小于利福平小于四环素小于红霉素和萘啶酸。培养48小时后去除抗生素时，在72小时仍有明显的增殖抑制作用。在96小时时，大多数培养物中出现了细菌再生长。在所测试的所有抗生素浓度下，萘啶酸的再生长率最高，其次是红霉素。每毫升米诺环素低于0.1微克、每毫升土霉素、四环素和强力霉素低于0.01微克时可观察到再生长。然而，每毫升去甲金霉素超过0.01微克时，去除抗生素后抑制作用可持续长达72小时。这些结果表明，在体外消除巨噬细胞中的瑞氏埃里希体方面，去甲金霉素比强力霉素、土霉素和米诺环素稍有效，而四环素和利福平效果较差。萘啶酸和红霉素无效。
