大肠杆菌β-D-半乳糖苷酶的交联制剂。
A crosslinked preparation of E. coli beta-D-galactosidase.
作者信息
Khare S K, Gupta M N
机构信息
Department of Chemistry, Indian Institute of Technology, New Delhi.
出版信息
Appl Biochem Biotechnol. 1987 Sep-Dec;16:1-13. doi: 10.1007/BF02798351.
PMID:3142355
Abstract
beta-D-Galactosidase from E. Coli was crosslinked using glutaraldehyde and two bisimidoesters. With glutaraldehyde and dimethyl adipimidate (DMA), it is possible to obtain preparations having higher activity than the native enzyme. Glutaraldehyde and DMA gave preparations showing enhanced thermal stability. The preparation crosslinked with DMA, when used for continuous hydrolysis of lactose in milk, was found to be significantly better than the native enzyme.
摘要
来自大肠杆菌的β-D-半乳糖苷酶使用戊二醛和两种双亚胺酯进行交联。使用戊二醛和己二酸二甲酯（DMA），有可能获得比天然酶活性更高的制剂。戊二醛和DMA得到的制剂表现出更高的热稳定性。当用与DMA交联的制剂用于牛奶中乳糖的连续水解时，发现其明显优于天然酶。
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