凝集素刺激的人T淋巴细胞中聚（ADP - 核糖）聚合酶基因表达的诱导依赖于蛋白质合成。

Induction of poly(ADP-ribose) polymerase gene expression in lectin-stimulated human T lymphocytes is dependent on protein synthesis.

作者信息

Menegazzi M, Gerosa F, Tommasi M, Uchida K, Miwa M, Sugimura T, Suzuki H

机构信息

Istituto di Chimica Biologica, Università di Verona, Italy.

出版信息

Biochem Biophys Res Commun. 1988 Oct 31;156(2):995-9. doi: 10.1016/s0006-291x(88)80942-2.

DOI:10.1016/s0006-291x(88)80942-2

PMID:3142469

Abstract

The poly(ADP-ribose) polymerase mRNA level in quiescent T lymphocytes was low, but was significantly higher than that in B lymphocytes or monocytes. When T lymphocytes were stimulated with phytohemagglutinin, a prompt increase in the mRNA level was observed from 4 hours after stimulation. The level of poly(ADP-ribose) polymerase mRNA reached a maximum in the late G1 phase about 1-2 days after lectin stimulation, and then decreased gradually returning to the basal level 10 days after lectin stimulation. Cycloheximide abrogated increase in poly(ADP-ribose) polymerase gene expression suggesting that a newly synthesized protein(s) was involved in poly(ADP-ribose) polymerase gene induction in lectin-stimulated T lymphocytes.

摘要

静止T淋巴细胞中的聚（ADP - 核糖）聚合酶mRNA水平较低，但明显高于B淋巴细胞或单核细胞中的水平。当用植物血凝素刺激T淋巴细胞时，从刺激后4小时开始观察到mRNA水平迅速增加。聚（ADP - 核糖）聚合酶mRNA水平在凝集素刺激后约1 - 2天的G1期后期达到最大值，然后逐渐下降，在凝集素刺激后10天恢复到基础水平。放线菌酮消除了聚（ADP - 核糖）聚合酶基因表达的增加，表明新合成的一种或多种蛋白质参与了凝集素刺激的T淋巴细胞中聚（ADP - 核糖）聚合酶基因的诱导。