Alves C S, Mourão P A
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brazil.
Atherosclerosis. 1988 Oct;73(2-3):113-24. doi: 10.1016/0021-9150(88)90032-9.
Aortic glycosaminoglycans were separated into fractions of increasing molecular weights containing heparan sulfate or chondroitin 4/6-sulfate + dermatan sulfate. When these fractions were added to plasma low density lipoproteins (LDL) in the presence of Ca2+, only chondroitin 4/6 sulfate + dermatan sulfate of high relative molecular weight produced turbidity. Treatment with testicular hyaluronidase abolished totally the formation of insoluble complex. When these glycosaminoglycans were applied to LDL-affinity columns in the presence of Ca2+, higher NaCl concentrations were necessary for the elution of the high relative molecular weight chondroitin sulfate. Heparan sulfate fractions did not produce turbidity when added to LDL solutions and were eluted from LDL-affinity columns at low NaCl concentrations. All these results suggest that besides the structure (or charge density), the molecular weight of the chondroitin sulfate chains is a relevant factor for the binding of this compound to LDL.
主动脉糖胺聚糖被分离成分子量递增的级分，其中包含硫酸乙酰肝素或硫酸软骨素4/6 +硫酸皮肤素。当在Ca2+存在的情况下将这些级分添加到血浆低密度脂蛋白（LDL）中时，只有高相对分子质量的硫酸软骨素4/6 +硫酸皮肤素产生浑浊。用睾丸透明质酸酶处理可完全消除不溶性复合物的形成。当在Ca2+存在的情况下将这些糖胺聚糖应用于LDL亲和柱时，洗脱高相对分子质量硫酸软骨素需要更高的NaCl浓度。硫酸乙酰肝素级分添加到LDL溶液中时不会产生浑浊，并在低NaCl浓度下从LDL亲和柱上洗脱下来。所有这些结果表明，除了结构（或电荷密度）外，硫酸软骨素链的分子量也是该化合物与LDL结合的一个相关因素。
