Mourão P A, Bracamonte C A
Atherosclerosis. 1984 Feb;50(2):133-46. doi: 10.1016/0021-9150(84)90017-0.
The interaction of glycosaminoglycans and proteoglycans with low density lipoproteins has been studied. Aortic and cartilaginous glycosaminoglycans are retained in LDL affinity columns and produce turbidity when added to LDL in presence of Ca2+. When extracted from whole aortic walls, dermatan sulfate is the glycosaminoglycan that shows greatest affinity for LDL. However, when using the glycosaminoglycans obtained by papain hydrolysis of the proteoglycans extracted from aorta, the binding affinity with LDL was similar for dermatan sulfate and chondroitin 4/6-sulfate. Removal of the protein core of the aortic or cartilaginous proteoglycans did not prevent interaction with LDL. However, treatment with testicular hyaluronidase abolished totally such interaction. When aortic glycosaminoglycans and proteoglycans were applied to LDL affinity columns in presence of Ca2+, a marked increase in the average molecular weight of the glycans found in the eluates of higher NaCl concentration was observed. This result suggests that the molecular weight of the glycosaminoglycans is a relevant factor for the binding of these compounds to LDL.
已对糖胺聚糖和蛋白聚糖与低密度脂蛋白的相互作用进行了研究。主动脉和软骨糖胺聚糖保留在低密度脂蛋白亲和柱中，当在钙离子存在的情况下添加到低密度脂蛋白中时会产生浑浊。从整个主动脉壁中提取时，硫酸皮肤素是对低密度脂蛋白显示出最大亲和力的糖胺聚糖。然而，当使用通过木瓜蛋白酶水解从主动脉中提取的蛋白聚糖获得的糖胺聚糖时，硫酸皮肤素和硫酸软骨素4/6-硫酸酯与低密度脂蛋白的结合亲和力相似。去除主动脉或软骨蛋白聚糖的蛋白核心并不能阻止与低密度脂蛋白的相互作用。然而，用睾丸透明质酸酶处理可完全消除这种相互作用。当在钙离子存在的情况下将主动脉糖胺聚糖和蛋白聚糖应用于低密度脂蛋白亲和柱时，观察到在较高氯化钠浓度洗脱液中发现的聚糖平均分子量显著增加。这一结果表明，糖胺聚糖的分子量是这些化合物与低密度脂蛋白结合的一个相关因素。
