Plant Ecology and Climate Change Science, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, U.P, India; Molecular Plant Virology Lab, Department of Botany, University of Lucknow, Lucknow, 226007, U.P, India.
Centre for Polymer Technology, Amity School of Applied Sciences, Amity University Haryana, Gurgaon, 122413, India.
Plant Physiol Biochem. 2019 Sep;142:460-471. doi: 10.1016/j.plaphy.2019.08.008. Epub 2019 Aug 13.
The present study is focused on the biogenic synthesis of AgNPs (B-AgNPs) using fruit extract of Phyllanthus emblica L. and its effect (0, 5, 10, 25, 50 mg/L concentrations) on early seedling growth of two wheat varieties (HD-2967 and DBW-17). The prepared silver nanoparticles were characterized with several techniques such as UV-Vis spectroscopy, powder X-ray diffraction as well as high-resolution transmission electron microscopy. The capping of AgNPs by phytochemicals was confirmed by Fourier transforms infrared (FT-IR) spectroscopy. B-AgNPs, chemically synthesized AgNPs, chemically synthesized AgNPs+10% fruit extract and AgNO salt were compared for phytotoxicity, based on growth parameters, ROS production, cytotoxicity assay and silver accumulation in two wheat varieties (HD-2967 and DBW-17). These effects were more pronounced in the variety HD-2967 over DBW-17 variety at 10 mg/L B-AgNPs exposure. Root cells viability of treated radicles was studied using Evans blue dye assay which suggest that 10 mg/L B-AgNPs was effective in promoting early seedling growth by decreasing ROS toxicity. Lower accumulation of Ag resulting in higher root cell viability than those of chemically synthesized AgNPs treated seedlings. The findings of the present study clearly indicate that phytochemicals capped AgNPs act as a growth promoter at lower concentrations by delivering a potent antioxidant during early seedling growth as compared to chemically synthesized AgNPs treated wheat seedlings.
本研究采用余甘子（Phyllanthus emblica L.）的果实提取物，通过生物法合成银纳米颗粒（B-AgNPs），并研究其对两种小麦品种（HD-2967 和 DBW-17）早期幼苗生长的影响（浓度分别为 0、5、10、25、50mg/L）。采用紫外可见分光光度计、粉末 X 射线衍射和高分辨率透射电子显微镜等技术对制备的银纳米颗粒进行了表征。通过傅里叶变换红外（FT-IR）光谱证实了银纳米颗粒被植物化学物质所包裹。基于生长参数、ROS 产生、细胞毒性测定和两种小麦品种（HD-2967 和 DBW-17）中银的积累，比较了 B-AgNPs、化学合成 AgNPs、化学合成 AgNPs+10%果提取物和 AgNO3 盐的植物毒性。在 10mg/L B-AgNPs 暴露下，HD-2967 品种的这些影响比 DBW-17 品种更为明显。用 Evans 蓝染料法研究了处理根尖的根细胞活力，结果表明 10mg/L B-AgNPs 通过降低 ROS 毒性有效促进了早期幼苗生长。与化学合成 AgNPs 处理的幼苗相比，B-AgNPs 较低的银积累导致更高的根细胞活力。本研究结果清楚地表明，与化学合成 AgNPs 处理的小麦幼苗相比，在较低浓度下，植物化学物质包裹的 AgNPs 通过在早期幼苗生长过程中提供有效的抗氧化剂，作为生长促进剂发挥作用。
