Laboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials, University of Sorocaba (UNISO), Rod. Raposo Tavares, km 92.5 - Vila Artura, Sorocaba, São Paulo, 18023-000, Brazil.
Laboratory of Environmental Nanotechnology, São Paulo State University (UNESP), Av. Três de Março 511, Sorocaba, São Paulo, 18087-180, Brazil.
BMC Plant Biol. 2022 May 24;22(1):255. doi: 10.1186/s12870-022-03638-1.
Biogenic metallic nanoparticles have been emerging as a promising alternative for the control of phytopathogens and as nanofertilizers. In this way, it is essential to investigate the possible impacts of these new nanomaterials on plants. In this study, the effects of soil contamination with biogenic silver (AgNPs) and iron (FeNPs) with known antifungal potential were investigated on morphological, physiological and biochemical parameters of soybean seedlings.
The exposure of plants/seedlings to AgNPs induced the reduction of root dry weight followed by oxidative stress in this organ, however, adaptive responses such as a decrease in stomatal conductance without impacts on photosynthesis and an increase in intrinsic water use efficiency were also observed. The seedlings exposed to FeNPs had shown an increase in the levels of oxygen peroxide in the leaves not accompanied by lipid peroxidation, and an increase in the expression of POD2 and POD7 genes, indicating a defense mechanism by root lignification.
Our results demonstrated that different metal biogenic nanoparticles cause different effects on soybean seedlings and these findings highlight the importance of investigating possible phytotoxic effects of these nanomaterials for the control of phytopathogens or as nanofertilizers.
生物成因的金属纳米粒子作为防治植物病原体的一种有前途的替代品和纳米肥料而出现。因此，有必要研究这些新材料对植物可能产生的影响。在这项研究中，研究了具有已知抗真菌潜力的生物成因银（AgNPs）和铁（FeNPs）对大豆幼苗形态、生理和生化参数的影响。
AgNPs 的暴露导致根干重减少，随后根发生氧化应激，但也观察到了适应反应，如气孔导度降低而光合作用不受影响，以及内在水分利用效率增加。暴露于 FeNPs 的幼苗叶片中过氧化氢水平升高，但没有脂质过氧化，POD2 和 POD7 基因的表达增加，表明根木质化的防御机制。
我们的结果表明，不同的金属生物成因纳米粒子对大豆幼苗产生不同的影响，这些发现强调了研究这些纳米材料作为防治植物病原体的纳米肥料或可能的植物毒性作用的重要性。
