Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan.
Department of Medical Technology, Yokkaichi Nursing and Medical Care University, Yokkaichi 512-8045, Japan.
Cells. 2022 May 7;11(9):1579. doi: 10.3390/cells11091579.
Wheat is vulnerable to numerous diseases; on the other hand, silver nanoparticles (AgNPs) exhibit a sterilizing action. To understand the combined effects of AgNPs with nicotinate and potassium nitrate (KNO) for plant growth and sterilization, a gel- and label-free proteomics was performed. Root weight was promoted by the treatment of AgNPs mixed with nicotinate and KNO. From a total of 5557 detected proteins, 90 proteins were changed by the mixture of AgNPs, nicotinate, and KNO; among them, 25 and 65 proteins increased and decreased, respectively. The changed proteins were mainly associated with redox and biotic stress in the functional categorization. By immunoblot analysis, the abundance of glutathione reductase/peroxiredoxin and pathogen-related protein three significantly decreased with the mixture. Furthermore, from the changed proteins, the abundance of starch synthase and lipoxygenase significantly increased and decreased, respectively. Through biochemical analysis, the starch contents increased with the mixture. The application of esculetin, which is a lipoxygenase inhibitor, increased the weight and length of the root. These results suggest that the AgNPs mixed with nicotinate and KNO cause positive effects on wheat seedlings by regulating pathogen-related protein and reactive-oxygen species scavenging. Furthermore, increasing starch and decreasing lipoxygenase might improve wheat growth.
小麦易患多种疾病；另一方面，纳米银颗粒（AgNPs）具有杀菌作用。为了了解 AgNPs 与烟酸和硝酸钾（KNO）联合使用对植物生长和杀菌的综合影响，进行了无凝胶和无标签的蛋白质组学研究。AgNPs 与烟酸和 KNO 混合处理促进了根重的增加。在总共检测到的 5557 种蛋白质中，AgNPs、烟酸和 KNO 的混合物改变了 90 种蛋白质；其中，分别有 25 种和 65 种蛋白质增加和减少。在功能分类中，这些变化的蛋白质主要与氧化还原和生物胁迫有关。通过免疫印迹分析，混合物中谷胱甘肽还原酶/过氧化物酶和与病原体相关的蛋白三的丰度显著降低。此外，从变化的蛋白质中，淀粉合酶和脂氧合酶的丰度分别显著增加和减少。通过生化分析，混合物中的淀粉含量增加。应用抑制脂氧合酶的七叶苷可增加根的重量和长度。这些结果表明，AgNPs 与烟酸和 KNO 混合使用通过调节与病原体相关的蛋白和活性氧清除来对小麦幼苗产生积极影响。此外，增加淀粉和减少脂氧合酶可能会改善小麦的生长。
