Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing, 100048, China; School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, Hubei, 430073, China; College of Food Science & Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, Hubei, 430073, China.
Carbohydr Polym. 2019 Nov 1;223:115065. doi: 10.1016/j.carbpol.2019.115065. Epub 2019 Jul 8.
Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function. Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process. The trehalose and whey protein isolate (WPI) ingredients in the cryoprotectants prolonged the dissolution time of Ca-alginate shell, which contributed to controlling the release of cells in the desired region. The dry ACFP capsules exhibited gradual release of L. plantarum cells in simulated intestinal fluid (SIF), 2.6 × 10 cfu/mL at 210 min. In addition, 0.1 g of the dry ACFP capsules showed the viable release amount of 3.3 × 10 cfu/mL after the storage of 160 days at 4 °C. The promising results provided a strategy of encapsulating probiotic cells to achieve long-term storage stability and enhanced controlled release behavior in simulated intestinal fluid in the meantime.
益生菌被认为对人类健康有益；然而，良好的储存稳定性是益生菌产品发挥功能的前提。在此，我们报告了一种制备海藻酸钙/冷冻保护剂/纤维素复合材料（ACFP）胶囊以保护植物乳杆菌细胞的方法，该方法在真空冷冻干燥过程中使细胞活力损失最小。冷冻保护剂中的海藻糖和乳清蛋白分离物（WPI）成分延长了海藻酸钙壳的溶解时间，有助于控制细胞在所需区域的释放。干燥的 ACFP 胶囊在模拟肠液（SIF）中表现出植物乳杆菌细胞的逐渐释放，210 分钟时达到 2.6×10cfu/mL。此外，在 4°C 下储存 160 天后，0.1g 干燥的 ACFP 胶囊显示出 3.3×10cfu/mL 的活菌释放量。这些有前景的结果为封装益生菌细胞提供了一种策略，以实现长期储存稳定性和同时在模拟肠液中增强的控制释放行为。
