On Stephen L W, Althaus Damien, Miller William G, Lizamore Darrell, Wong Samuel G L, Mathai Anso J, Chelikani Venkata, Carter Glen P
Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand.
Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, CA, United States.
Front Microbiol. 2019 Aug 5;10:1802. doi: 10.3389/fmicb.2019.01802. eCollection 2019.
A wide range of species have been described from shellfish in various countries but their presence has not been investigated in Australasia, in which shellfish are a popular delicacy. Since several arcobacters are considered to be emerging pathogens, we undertook a small study to evaluate their presence in several different shellfish, including greenshell mussels, oysters, and abalone (paua) in New Zealand. , a species associated with human gastroenteritis, was the only species isolated, from greenshell mussels. Whole-genome sequencing revealed a range of genomic traits in these strains that were known or associated virulence factors. Furthermore, we describe the first putative virulence plasmid in , containing lytic, immunoavoidance, adhesion, antibiotic resistance, and gene transfer traits, among others. Complete genome sequence determination using a combination of long- and short-read genome sequencing strategies, was needed to identify the plasmid, clearly identifying its benefits. The potential for plasmids to disseminate virulence traits among and other species warrants further consideration by researchers interested in the risks to public health from these organisms.
在各个国家，已从贝类中描述出多种物种，但在贝类是一种受欢迎美食的澳大拉西亚地区，尚未对它们的存在情况进行调查。由于几种弓形杆菌被认为是新出现的病原体，我们开展了一项小型研究，以评估它们在新西兰的几种不同贝类中的存在情况，这些贝类包括绿壳贻贝、牡蛎和鲍鱼（新西兰鲍）。与人类肠胃炎相关的一种物种是从绿壳贻贝中分离出的唯一物种。全基因组测序揭示了这些菌株中一系列已知或与毒力因子相关的基因组特征。此外，我们描述了该物种中首个假定的毒力质粒，它含有裂解、免疫逃避、黏附、抗生素抗性和基因转移等特征。需要使用长读长和短读长基因组测序策略相结合的方法来确定完整的基因组序列，以鉴定该质粒，明确其益处。质粒在该物种和其他物种之间传播毒力特征的可能性值得关注这些生物体对公众健康风险的研究人员进一步考虑。
