与大鼠肝细胞相比，在特定条件下原代培养的小鼠肝细胞的活跃增殖。
Active proliferation of mouse hepatocytes in primary culture under defined conditions as compared to rat hepatocytes.
作者信息
Sawada N, Lee G H, Mochizuki Y, Ishikawa T
机构信息
Department of Experimental Pathology, Cancer Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1988 Sep;79(9):983-8. doi: 10.1111/j.1349-7006.1988.tb00064.x.
Abstract
Mouse and rat hepatocytes were investigated for replicative potential in primary culture under defined conditions. Mouse hepatocytes replicated at least twice or three times by the 7th day of culture in response to EGF, while only a half of rat hepatocytes replicated once. Thus a far greater potential for proliferation was demonstrated by mouse than rat cells, although the mouse cells ceased dividing by the 10th day of culture. Immunocytochemical examination revealed that the cells produced both albumin and alpha-fetoprotein. Ultrastructural investigation revealed that these cells were immature hepatocytes in nature, based on the presence of microbodies with nucleoids. Thus, primary cultures of mouse hepatocytes seem to be a useful system for studying proliferation and transformation of hepatocytes in vitro.
摘要
在特定条件下，对原代培养中的小鼠和大鼠肝细胞的增殖潜力进行了研究。在培养的第7天，小鼠肝细胞在表皮生长因子（EGF）的作用下至少复制了两到三次，而大鼠肝细胞只有一半进行了一次复制。因此，尽管小鼠细胞在培养的第10天停止了分裂，但与大鼠细胞相比，小鼠细胞显示出了更大的增殖潜力。免疫细胞化学检查显示，这些细胞能产生白蛋白和甲胎蛋白。超微结构研究表明，基于含拟核微体的存在，这些细胞本质上是未成熟的肝细胞。因此，小鼠肝细胞的原代培养似乎是体外研究肝细胞增殖和转化的一个有用系统。
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