Quorum-sensing based small RNA regulation for dynamic and tuneable gene expression.
作者信息
Bao Shao-Heng, Li Wen-Ying, Liu Chang-Jun, Zhang Dong-Yi, Meng Er
机构信息
Research Center of Biological Information, College of Arts and Sciences, National University of Defense Technology, Changsha, Hunan, People's Republic of China.
School of Life Sciences, Hunan University of Science and Technology, Xiangtan, Hunan, People's Republic of China.
出版信息
Biotechnol Lett. 2019 Oct;41(10):1147-1154. doi: 10.1007/s10529-019-02719-w. Epub 2019 Aug 19.
OBJECTIVES
Developing a dynamic regulation strategy is an essential step in establishing an automatic control system for manipulating metabolic fluxes and cellular behaviors. To broaden the extent of the application, a system that can generally control any gene of interest is demanded.
RESULTS
Through characterization and optimization, the strategy repressed the immediate expression incrementally from 0 to 90% during culturing. Moreover, by changing single base pair in the lux box of the P promoter, the degree of repression of the target genomic gene was tuned to a difference of 70%. This strategy is expected to control metabolic flux without disrupting cell growth.
CONCLUSIONS
We engineered bacterial small RNA to develop a pathway-independent strategy that can dynamically repress the expression of any gene at the posttranscription level.
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