快速切换高压多孔尖端电喷雾电离质谱法快速检测掺杂草药膳食补充剂中的抗风湿药物。
Fast-switching high-voltage porous-tip electrospray ionization mass spectrometry for rapid detection of antirheumatic drugs in adulterated herbal dietary supplements.
机构信息
Institute of Mass Spectrometry and Atmospheric Environment, Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Jinan University, Guangzhou, 510632, China.
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, 510632, China.
出版信息
Rapid Commun Mass Spectrom. 2019 Dec 30;33(24):1877-1883. doi: 10.1002/rcm.8559.
RATIONALE
Herbal dietary supplements (HDSs) adulterated with undeclared synthetic drugs can lead to serious health problems METHODS: A fast-switching positive/negative high-voltage (+/- HV) was developed to apply on electrospray ionization mass spectrometry (ESI-MS) with porous tips for rapid screening of five antirheumatic drugs in antirheumatic HDSs. The fast-switching (switch-time: 100 ms) negative and positive ions were alternately generated to perform full-MS and tandem-MS analysis, providing an effective method for rapid detection of analytes in whichever mode of detection was most suitable (negative or positive ion mode). The use of different tips and solvents was also optimized in this work.
RESULTS
The limits of detection of the five antirheumatic drugs were found to be less than 0.1 ng/g (S/N > 3). The reproducibility of the five drugs was measured to be 10.0-23.3% (n = 5). A single sample analysis could be completed within 1 min. Rapid screening of a total of 28 real HDS samples collected from the market was examined by the fast-switching HV substrate-tip ESI-MS method, and the screening result was further validated by conventional liquid chromatography/mass spectrometry.
CONCLUSIONS
Overall, our results demonstrated that fast-switching HV substrate-tip ESI-MS is a rapid, reliable, and effective method for simultaneous screening of various analytes in complex samples.
原理
含有未申报合成药物的草药膳食补充剂（HDS）可能导致严重的健康问题。
方法
开发了一种快速切换正/负高压（+/- HV），应用于带有多孔尖端的电喷雾电离质谱（ESI-MS），用于快速筛选抗风湿 HDS 中的五种抗风湿药物。快速切换（切换时间：100 ms）的正、负离子交替产生，进行全质谱和串联质谱分析，为在最适合的检测模式（负离子或正离子模式）中快速检测分析物提供了有效的方法。在这项工作中，还优化了不同尖端和溶剂的使用。
结果
五种抗风湿药物的检测限均低于 0.1 ng/g（S/N > 3）。五种药物的重现性测量值为 10.0-23.3%（n = 5）。单个样品的分析可以在 1 分钟内完成。通过快速切换 HV 基底尖端 ESI-MS 方法对从市场上采集的总共 28 个真实 HDS 样本进行了快速筛选，并通过常规液相色谱/质谱对筛选结果进行了进一步验证。
结论
总体而言，我们的结果表明，快速切换 HV 基底尖端 ESI-MS 是一种快速、可靠、有效的方法，可用于同时筛选复杂样品中的各种分析物。
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