Ma Yan, Wang Pei, Gu Zhenxin, Tao Yang, Shen Chang, Zhou Yulin, Han Yongbin, Yang Runqiang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Food Chem X. 2019 Apr 5;2:100023. doi: 10.1016/j.fochx.2019.100023. eCollection 2019 Jun 30.
In this study, in order to investigate the role of Ca in GABA signal transduction involved in phenolics accumulation in barley seedlings under NaCl stress, the seedlings were treated with exogenous GABA and its synthesis inhibitor, 3-mercaplopropionic acid (3-MP), as well as Ca channel blockers La, Ca chelator EGTA, and Ca release channel inhibitor 2-aminoethoxydiphenyl borate (2-APB). The results showed that GABA significantly enhanced phenolics, calcium and calmodulin content. It also induced Ca influx in barley root tips cells, and altered the distribution of Ca, making calcium precipitates more uniform and intensive. While, 3-MP treatment led to opposite changes, which suggested that GABA was essential for calcium content increase. In addition, accumulation of phenolics was inhibited by LaCl, EGTA and 2-APB treatments, and this inhibition could be alleviated partly by exogenous GABA. Taken together, Ca was involved in GABA signal transduction for phenolics accumulation in barley seedlings under NaCl stress.
在本研究中，为了探究钙在NaCl胁迫下大麦幼苗酚类物质积累所涉及的γ-氨基丁酸（GABA）信号转导中的作用，用外源GABA及其合成抑制剂3-巯基丙酸（3-MP），以及钙通道阻滞剂镧（La）、钙螯合剂乙二醇双四乙酸（EGTA）和钙释放通道抑制剂2-氨基乙氧基二苯硼酸（2-APB）处理幼苗。结果表明，GABA显著提高了酚类物质、钙和钙调蛋白的含量。它还诱导了大麦根尖细胞中的钙内流，并改变了钙的分布，使钙沉淀更均匀、更密集。而3-MP处理则导致相反的变化，这表明GABA对钙含量的增加至关重要。此外，LaCl、EGTA和2-APB处理抑制了酚类物质的积累，而外源GABA可部分缓解这种抑制作用。综上所述，钙参与了NaCl胁迫下大麦幼苗酚类物质积累的GABA信号转导。
