Jiao Caifeng, Wang Pei, Yang Runqiang, Tian Lu, Gu Zhenxin
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
J Agric Food Chem. 2016 Nov 9;64(44):8282-8288. doi: 10.1021/acs.jafc.6b02633. Epub 2016 Oct 28.
In this study, to investigate the role of inositol 1,4,5-trisphosphate (IP3) in nitric oxide-guanosine 3',5'-cyclic monophosphate (NO-cGMP)-induced isoflavone accumulation in soybean sprouts under UV-B radiation, the sprouts were treated with donors and inhibitors of NO and cGMP as well as IP3 inhibitor. Results showed that NO, with cGMP as a second messenger, stimulates IP3 accumulation under UV-B radiation. Consistent with the increase in IP3 content, the up-regulation of gene and protein expression of phosphoinositide-specific phospholipase C (PI-PLC) in response to sodium nitroprusside (SNP) (exogenous NO donor) and 8-Br-cGMP (cGMP analogue) was also observed. In addition, protein kinase G (PKG) participated in NO-cGMP-induced IP3 production. IP3 induced by the NO-cGMP pathway was involved in isoflavone synthesis by elevating the activity and gene and protein expressions of chalcone synthase (CHS) and isoflavone synthase (IFS). Overall, IP3 mediates NO-cGMP-induced isoflavone accumulation in soybean sprouts under UV-B stress.
在本研究中，为了探究1,4,5-三磷酸肌醇（IP3）在紫外线B（UV-B）辐射下一氧化氮-3',5'-环磷酸鸟苷（NO-cGMP）诱导的大豆芽异黄酮积累中的作用，用NO、cGMP的供体和抑制剂以及IP3抑制剂处理豆芽。结果表明，在UV-B辐射下，以cGMP作为第二信使的NO刺激IP3积累。与IP3含量的增加一致，还观察到响应硝普钠（SNP，外源性NO供体）和8-溴-cGMP（cGMP类似物）时，磷脂酰肌醇特异性磷脂酶C（PI-PLC）的基因和蛋白表达上调。此外，蛋白激酶G（PKG）参与了NO-cGMP诱导的IP3产生。由NO-cGMP途径诱导的IP3通过提高查尔酮合酶（CHS）和异黄酮合酶（IFS）的活性以及基因和蛋白表达而参与异黄酮合成。总体而言，IP3介导UV-B胁迫下NO-cGMP诱导的大豆芽异黄酮积累。
