比较转录组分析揭示了采后草莓在高环境温度下果实变色的机制。

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature.

作者信息

Zhang Liping, Wang Lu, Zeng Xiangguo, Chen Ruixu, Yang Shuzhen, Pan Siyi

机构信息

Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.

Economic Crop Research Institute, Hubei Academy of Agricultural Sciences, PR China.

出版信息

Food Chem X. 2019 Apr 27;2:100025. doi: 10.1016/j.fochx.2019.100025. eCollection 2019 Jun 30.

The aim of this study was to examine both physiological and molecular evidences related to fruit discoloration in postharvest strawberries under high ambient temperature. The results showed the total anthocyanin and their main components in the strawberries under 35 °C were greatly increased due to the significant up-regulations of anthocyanin biosynthetic genes and transportation genes. High ambient temperature greatly improved the activities of peroxidase (POD) and enhanced gene expressions of , and . At the same time, high storage temperature activated laccase genes expression including and , which was closely related to anthocyanin degradation. Levels of reactive oxygen species (ROS) metabolism were also increased under high ambient temperature at transcript levels. Therefore, we concluded that high ambient temperature could enhance anthocyanin accumulation and degradation at the same time, which maybe the main reasons for the fruits discoloration of postharvest strawberries under high ambient temperature.

本研究旨在探究与采后草莓在高环境温度下果实变色相关的生理和分子证据。结果表明，35°C下草莓中的总花青素及其主要成分大幅增加，这是由于花青素生物合成基因和转运基因的显著上调所致。高环境温度极大地提高了过氧化物酶（POD）的活性，并增强了、和的基因表达。同时，高贮藏温度激活了包括和在内的漆酶基因表达，这与花青素降解密切相关。在转录水平上，高环境温度下活性氧（ROS）代谢水平也有所增加。因此，我们得出结论，高环境温度可能同时增强了花青素的积累和降解，这可能是采后草莓在高环境温度下果实变色的主要原因。