去除果蝇胚胎4D电影中卵黄膜自发荧光的自动化宏方法

Automated Macro Approach to Remove Vitelline Membrane Autofluorescence in Drosophila Embryo 4D Movies.

作者信息

Boix-Fabrés Jaume, Karkali Katerina, Martín-Blanco Enrique, Rebollo Elena

机构信息

Molecular Imaging Platform, Molecular Biology Institute of Barcelona, Spanish National Research Council (CSIC), Barcelona, Spain.

Molecular Biology Institute of Barcelona (IBMB-CSIC), Barcelona, Spain.

出版信息

Methods Mol Biol. 2019;2040:155-175. doi: 10.1007/978-1-4939-9686-5_9.

DOI:10.1007/978-1-4939-9686-5_9

PMID:31432480

Abstract

This chapter provides an ImageJ/Fiji automated macro approach to remove the vitelline membrane autofluorescence in live Drosophila embryo movies acquired in a 4D (3D plus time) fashion. The procedure consists in a segmentation pipeline that can cope with different relative intensities of the vitelline membrane autofluorescence, followed by a developed algorithm that adjusts the extracted outline selection to the shape deformations that naturally occur during Drosophila embryo development. Finally, the fitted selection is used to clear the external glowing halo that, otherwise, would obscure the visualization of the internal embryo labeling upon projection or 3D rendering.

摘要

本章介绍了一种使用ImageJ/Fiji的自动宏方法，用于去除以4D（3D加时间）方式获取的活果蝇胚胎电影中的卵黄膜自发荧光。该过程包括一个分割管道，它可以处理卵黄膜自发荧光的不同相对强度，随后是一种开发的算法，该算法可将提取的轮廓选择调整为果蝇胚胎发育过程中自然发生的形状变形。最后，拟合的选择用于清除外部发光光晕，否则在投影或3D渲染时会遮挡内部胚胎标记的可视化。