School of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.
Department of Cellular Biology, University of Georgia, Athens, GA, USA.
Nat Commun. 2021 May 25;12(1):3148. doi: 10.1038/s41467-021-23449-6.
Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.
结构光照明显微镜能够以亚衍射分辨率进行活细胞成像。然而，光学像差会导致分辨率降低，并在结构光照明显微镜中产生伪影，从而使该技术在厚度超过单个细胞的样本中无法使用。在这里，我们报告了自适应光学与结构光照明显微镜的结合，实现了在秀丽隐杆线虫深度为 80μm 处具有 150nm 侧向和 570nm 轴向分辨率的成像。我们证明了自适应光学可以提高三维分辨率，特别是在轴向方向上，并且减少了伪影，成功地在各种生物样本中实现了 3D-Structured Illumination Microscopy。
