直接体细胞胚胎发生在 Carica papaya L. 基因型中用于遗传修饰目的。

Direct Somatic Embryogenesis in Carica papaya L. Genotypes for Genetic Modification Purposes.

机构信息

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.

Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Ixtacuaco, Veracruz, Mexico.

出版信息

Methods Mol Biol. 2024;2827:279-290. doi: 10.1007/978-1-0716-3954-2_19.

DOI:10.1007/978-1-0716-3954-2_19

PMID:38985277

Abstract

This chapter presents an efficient protocol for regenerating Carica papaya plants via somatic embryogenesis from immature zygotic embryos from economically important papaya genotypes. To achieve regenerated plants from somatic embryos, in the present protocol, four induction cycles are required, followed by one multiplication cycle and one regeneration cycle. With this optimized protocol, 80% of somatic embryos can be obtained in only 3.5 months. At this stage, calli containing more than 50% globular structures can be used for transformation (via agrobacterium, biobalistics, or any other transformation method). Once transformed, calli can be transferred to the following steps (multiplication, elongation, maturation, rooting, and ex vitro acclimatization) to regenerate a transformed somatic embryo-derived full plant.

摘要

本章介绍了一种从经济上重要的木瓜基因型的未成熟合子胚通过体细胞胚胎发生再生木瓜植株的有效方案。为了从体细胞胚获得再生植株，在本方案中，需要进行四个诱导循环，然后进行一个增殖循环和一个再生循环。使用这种优化的方案，仅需 3.5 个月即可获得 80%的体细胞胚。在这个阶段，含有超过 50%球状结构的愈伤组织可用于转化（通过农杆菌、生物弹道或任何其他转化方法）。转化后，可将愈伤组织转移到以下步骤（增殖、伸长、成熟、生根和离体驯化），以再生转化的体细胞胚衍生的完整植株。