Zheng Zheng, Xu Dan, Shi Keqing, Chen Minfeng, Lu Fan
School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang 325000, China.
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
Aging (Albany NY). 2019 Aug 20;11(16):6153-6174. doi: 10.18632/aging.102178.
Uveal melanomas are the most common primary intraocular malignant tumors in adults, associated with a high metastasis rate and a low 5-year survival rate. It is a clinic urgency and importance to identify prognostic factors for UVMs.
55 aberrantly methylated sites of miRNAs and 47 aberrantly methylated sites of lncRNAs were observed between Alive < 2 years group and Alive > 2 years group of UVMs. Two prognostic classifiers were generated. For 13- miRNAs-CpG-classifier, the AUC were 0.958, 0.848 and 0.824 at 1 year, 2 years and 3 years, respectively. For 9- lncRNAs-CpG-classifier, the AUC were 0.943, 0.869 and 0.866 at 1 year, 2 years and 3 years, respectively.
The correlation between genome-wide DNA methylation patterns of miRNAs and lncRNAs and the overall survival in UVMs were identified in this study. This novel finding shed new light on developing biomarkers of prognosis for UVMs.
DNA methylation profiles of noncoding miRNAs and lncRNAs for UVMs were accessed from The Cancer Genome Atlas. Then the prognostic value was analyzed by least absolute shrinkage and selection operator method Cox regression and tested by Time-dependent Receiver Operating Characteristic curve.
葡萄膜黑色素瘤是成人最常见的原发性眼内恶性肿瘤，具有高转移率和低5年生存率。识别葡萄膜黑色素瘤的预后因素具有临床紧迫性和重要性。
在葡萄膜黑色素瘤存活<2年组和存活>2年组之间观察到55个miRNA的异常甲基化位点和47个lncRNA的异常甲基化位点。生成了两个预后分类器。对于13-miRNA-CpG分类器，1年、2年和3年时的AUC分别为0.958、0.848和0.824。对于9-lncRNA-CpG分类器，1年、2年和3年时的AUC分别为0.943、0.869和0.866。
本研究确定了miRNA和lncRNA的全基因组DNA甲基化模式与葡萄膜黑色素瘤总生存之间的相关性。这一新发现为开发葡萄膜黑色素瘤的预后生物标志物提供了新线索。
从癌症基因组图谱获取葡萄膜黑色素瘤非编码miRNA和lncRNA的DNA甲基化谱。然后通过最小绝对收缩和选择算子法Cox回归分析预后价值，并通过时间依赖性受试者工作特征曲线进行检验。
