Zhuo Zhen-Jian, Zhang Ruizhong, Zhang Jiao, Zhu Jinhong, Yang Tianyou, Zou Yan, He Jing, Xia Huimin
Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzho, Guangdong 510623, China.
School of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China.
Aging (Albany NY). 2018 Mar 27;10(3):481-491. doi: 10.18632/aging.101406.
Neuroblastoma is the third most common childhood cancer after leukemias and cancer of the central nervous system. Long noncoding RNA polymorphisms have been shown to confer cancer susceptibility; however, their roles in the genetic predisposition to neuroblastoma remain unclarified. To answer this question, we genotyped two polymorphisms, rs7158663 G>A and rs4081134 G>A, in 392 neuroblastoma children and 783 controls by TaqMan method. The results showed that neither single locus nor the combination analysis supported an association between polymorphism and neuroblastoma risk. Interestingly, we found that subjects carrying rs4081134 AG/AA genotypes significantly tended to develop neuroblastoma among subgroups with age >18 month (adjusted OR=1.36, 95% CI=1.01-1.84) and clinical stage III+IV disease (adjusted OR=1.47, 95% CI=1.08-1.99), when compared with reference group. In the combined analysis of polymorphisms, we found that carriers of 2 risk genotypes were more likely to have higher risk of developing neuroblastoma than those with 0-1 risk genotype among children more than 18 months of age (adjusted OR=1.36, 95% CI=1.01-1.84, =0.042), and with clinical stages III+IV disease (adjusted OR=1.47, 95% CI=1.08-2.00, =0.014). Our data suggest as a weak-effect neuroblastoma susceptibility gene. Well-designed studies with large sample studies are needed to further validate this finding.
神经母细胞瘤是继白血病和中枢神经系统癌症之后第三常见的儿童癌症。长链非编码RNA多态性已被证明与癌症易感性有关；然而，它们在神经母细胞瘤遗传易感性中的作用仍不清楚。为了回答这个问题，我们采用TaqMan方法对392例神经母细胞瘤患儿和783例对照者进行了两个多态性位点rs7158663 G>A和rs4081134 G>A的基因分型。结果表明，无论是单一位点分析还是联合分析，均不支持多态性与神经母细胞瘤风险之间存在关联。有趣的是，我们发现，与参照组相比，携带rs4081134 AG/AA基因型的受试者在年龄>18个月的亚组（校正OR=1.36，95%CI=1.01-1.84）和临床III+IV期疾病亚组（校正OR=1.47，95%CI=1.08-1.99）中发生神经母细胞瘤的倾向明显更高。在多态性联合分析中，我们发现，在18个月以上的儿童中（校正OR=1.36，95%CI=1.01-1.84，P=0.042）以及临床III+IV期疾病患儿中（校正OR=1.47，95%CI=1.08-2.00，P=0.014），携带2种风险基因型的个体比携带0-1种风险基因型的个体发生神经母细胞瘤的风险更高。我们的数据表明，[此处原文可能缺失相关基因名称]是一种弱效应的神经母细胞瘤易感基因。需要开展设计良好的大样本研究来进一步验证这一发现。
