Baloji Gugulothu, Pasham Shobharani, Mahankali Vinodha, Garladinne Mallikarjuna, Ankanagari Srinivas
Department of Genetics and Biotechnology, Osmania University,Hyderabad (T.S) - 500 007,India.
Plant Molecular Biology Laboratory,Agri Biotech Foundation,Rajendra Nagar,Hyderabad (T.S) 500 030,India.
Bioinformation. 2019 Feb 28;15(2):131-138. doi: 10.6026/97320630015131. eCollection 2019.
The prevailing abiotic stresses, especially heat stress is of great significance on the growth of plants, yield and distribution. In the conditions of heat stress, plants modulate protein processes leading to development of heat tolerance. Of such proteins, the molecular chaperone functions of HSP70/HSC70 proteins are important where their enhanced expression positively correlates with the acquisition of heat tolerance. The key players in the regulation of such tailored protein responses of plants to heat stress are the phytohormones. In the present study, phytohormone mediated interaction of Pennisetum glaucum HSC70 (PgHSC70) protein was performed through docking studies involving sequence analysis, 3D modeling and model evaluation. In silico analysis has shown better interaction and good binding energy of PgHSC70 with the phytohormone brassinolide. Furthermore, the predicted structural information can be helpful for future studies on role of interaction between HSC70 and brassinolide in heat tolerance.
当前的非生物胁迫，尤其是热胁迫对植物的生长、产量和分布具有重要意义。在热胁迫条件下，植物会调节蛋白质过程以形成耐热性。在这类蛋白质中，HSP70/HSC70蛋白的分子伴侣功能很重要，其表达增强与耐热性的获得呈正相关。植物对热胁迫这种特定蛋白质反应调节的关键因素是植物激素。在本研究中，通过涉及序列分析、三维建模和模型评估的对接研究，进行了植物激素介导的黍稷HSC70（PgHSC70）蛋白相互作用研究。计算机模拟分析表明，PgHSC70与植物激素油菜素内酯具有更好的相互作用和良好的结合能。此外，预测的结构信息有助于未来研究HSC70与油菜素内酯之间的相互作用在耐热性中的作用。
