Department of Biotechnology, Faculty of Biological Sciences, University of Malakand, Chakdara, KPK, Pakistan.
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China.
Environ Sci Pollut Res Int. 2019 Oct;26(29):30333-30347. doi: 10.1007/s11356-019-06206-7. Epub 2019 Aug 21.
An in vitro study was conducted to evaluate the effects of thidiazuron (TDZ) growth regulator and magnesium oxide (MgO) nanoparticles on radish (Raphanus sativus L.) under lead (Pb) stress. Effects of TDZ and MgO on seed germination, growth, biomass, total phenolics and flavonoids, antioxidant potential, and Pb phytoaccumulation in different plant parts were assessed. Nanoparticles of MgO were synthesized with leaf extract of Sageretia thea (Osbeck) plant. Thidiazuron and MgO nanoparticles were added to growth media in individual and in combinations. Lead (50 mg L) was added to growth media. Thidiazuron and MgO nanoparticles increased plant growth, phenolic and flavonoid contents, free radical scavenging activity, and lead phytoaccumulation. The increase was highly significant in TDZ and MgO nanoparticle combination treatments (T5, T6). Treatment (T6) showed a sixfold increase in Pb accumulation (1721.73 ± 17.4 μg g dry biomass) as compared to control (274.29 ± 4.23 μgg). Total phenolic and dry biomass showed significantly positive correlation in leaves (R = 0.73), stem (R = 0.58), and roots (R = 0.72). The correlation of Pb accumulation and phenolic contents was significantly positive in root (R = 0.80), stem (R = 0.92), and leaves (R = 0.69). Flavonoid showed a positive correlation with dry biomass and Pb accumulation. Antioxidant activity was highly increased in leaves followed by stem and root. Findings show that TDZ in combination with MgO nanoparticles can play a significant role in secondary metabolite production and Pb phytoaccumulation.
一项体外研究评估了噻二唑脲（TDZ）生长调节剂和氧化镁（MgO）纳米粒子在铅（Pb）胁迫下对萝卜（Raphanus sativus L.）的影响。评估了 TDZ 和 MgO 纳米粒子对种子发芽、生长、生物量、总酚和类黄酮、抗氧化潜力以及不同植物部位 Pb 植物累积的影响。MgO 纳米粒子是用 Sageretia thea（Osbeck）植物的叶提取物合成的。将 TDZ 和 MgO 纳米粒子单独和组合添加到生长培养基中。向生长培养基中添加铅（50mg L）。TDZ 和 MgO 纳米粒子增加了植物生长、酚类和类黄酮含量、自由基清除活性和铅植物累积。TDZ 和 MgO 纳米粒子组合处理（T5、T6）的增加非常显著。处理（T6）的 Pb 积累量增加了六倍（1721.73±17.4μg g 干生物量），而对照（274.29±4.23μg g）。叶片（R=0.73）、茎（R=0.58）和根（R=0.72）中总酚和干生物量呈显著正相关。根（R=0.80）、茎（R=0.92）和叶（R=0.69）中 Pb 积累与酚含量的相关性呈显著正相关。类黄酮与干生物量和 Pb 积累呈正相关。叶片中抗氧化活性显著增加，其次是茎和根。研究结果表明，TDZ 与 MgO 纳米粒子联合使用可以在次生代谢产物产生和 Pb 植物累积方面发挥重要作用。
