Hussain Mubashir, Raja Naveed Iqbal, Mashwani Zia-Ur-Rehman, Iqbal Muhammad, Sabir Sidra, Yasmeen Farhat
Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
3 Biotech. 2017 Jun;7(2):101. doi: 10.1007/s13205-017-0741-6. Epub 2017 May 30.
In the recent years, nanotechnology has attained much attention in the every field of science. The synthesis, characterisation and applications of metallic nanoparticles (MNPs) have become an important branch of nanotechnology. In the current study, MNPs were synthesised through polyols process and applied in vitro to study their effect on medicinally important plant : Artemisia absinthium. The current study strives to check the effect of MNPs, i.e., Ag, Cu and Au on seed germination, root and shoot length, seedling vigour index (SVI) and biochemical profiling in A. absinthium. The seeds were inoculated on MS medium supplemented with various combinations of MNPs suspension. The seed germination was greatly influenced upon the application of MNPs and was recorded highest for the silver nanoparticles (AgNPs) suspensions. The best result for seed germination (98.6%) was obtained in MS medium supplemented with AgNPs as compared to control (92.9%) and other nanoparticles, i.e., copper (69.6%) and gold (56.5%), respectively, after 35 days of inoculation. Significant results were obtained for root length, shoot length and SVI in response to application of AgNPs as compared to copper nanoparticles (CuNPs) and gold nanoparticles (AuNPs). These nanoparticles (NPs) could induce stress in plants by deploying the endogenous mechanism. In response to these stresses, plants produce various defence compounds. Total phenolic content (TPC) and total flavonoid content (TFC) were significant in the MS medium supplemented with AgNPs as compared to other NPs, while DPPH radical scavenging assay (RSA) was highest in AuNPs treated plantlets. The MNPs showed higher toxicity level and enhanced secondary metabolites production, total phenolic content, total flavonoid content, antioxidant activity, superoxide dismutase (SOD) activity and total protein content.
近年来，纳米技术在各个科学领域都备受关注。金属纳米粒子（MNPs）的合成、表征及应用已成为纳米技术的一个重要分支。在本研究中，通过多元醇法合成了MNPs，并在体外研究其对药用重要植物——苦艾的影响。本研究旨在检测MNPs（即银、铜和金）对苦艾种子萌发、根和茎长度、幼苗活力指数（SVI）及生化分析的影响。将种子接种在添加了不同组合MNPs悬浮液的MS培养基上。MNPs的应用对种子萌发有很大影响，银纳米粒子（AgNPs）悬浮液的种子萌发率最高。接种35天后，添加AgNPs的MS培养基中种子萌发的最佳结果为98.6%，相比之下，对照组为92.9%，其他纳米粒子，即铜（69.6%）和金（56.5%）。与铜纳米粒子（CuNPs）和金纳米粒子（AuNPs）相比，AgNPs处理后根长、茎长和SVI均有显著结果。这些纳米粒子（NPs）可通过内源性机制在植物中诱导胁迫。作为对这些胁迫的响应，植物会产生各种防御化合物。与其他NPs相比，添加AgNPs的MS培养基中总酚含量（TPC）和总黄酮含量（TFC）显著，而在AuNPs处理的幼苗中DPPH自由基清除率（RSA）最高。MNPs显示出较高的毒性水平，并增强了次生代谢产物的产生、总酚含量、总黄酮含量、抗氧化活性、超氧化物歧化酶（SOD）活性和总蛋白含量。
