State Key Laboratory of Microbial Technology , Shandong University , Qingdao 266237 , People's Republic of China.
Institute of Marine Science and Technology , Shandong University , Qingdao 266237 , People's Republic of China.
Anal Chem. 2019 Sep 17;91(18):11981-11986. doi: 10.1021/acs.analchem.9b02875. Epub 2019 Sep 4.
Sulfane sulfur has been recognized as a common cellular component, participating in regulating enzyme activities and signaling pathways. However, the quantification of total sulfane sulfur in biological samples is still a challenge. Here, we developed a method to address the need. All tested sulfane sulfur reacted with sulfite and quantitatively converted to thiosulfate when heated at 95 °C in a solution of pH 9.5 for 10 min. The assay condition was also sufficient to convert total sulfane sulfur in biological samples to thiosulfate for further derivatization and quantification. We applied the method to detect sulfane sulfur contents at different growth phases of bacteria, yeast, mammalian cells, and zebrafish. Total sulfane sulfur contents in all of them increased in the early stage, kept at a steady state for a period, and declined sharply in the late stage of the growth. Sulfane sulfur contents varied in different species. For , growth media also affected the sulfane sulfur contents. Total sulfane sulfur contents from different organs of mouse and shrimp were also detected, varying from 1 to 10 nmol/(mg of protein). Thus, the new method is suitable for the quantification of total sulfane sulfur in biological samples.
硫氢化物硫已被认为是一种常见的细胞成分，参与调节酶活性和信号通路。然而，生物样品中总硫氢化物硫的定量仍然是一个挑战。在这里，我们开发了一种方法来满足这一需求。所有测试的硫氢化物硫在 pH 值为 9.5 的溶液中于 95°C 加热 10 分钟时与亚硫酸盐反应，并定量转化为硫代硫酸盐。该测定条件也足以将生物样品中的总硫氢化物硫转化为硫代硫酸盐，以进一步衍生化和定量。我们应用该方法检测了细菌、酵母、哺乳动物细胞和斑马鱼不同生长阶段的硫氢化物硫含量。它们的总硫氢化物硫含量在生长的早期阶段增加，在一段时间内保持稳定，然后在后期急剧下降。不同物种的硫氢化物硫含量不同。对于，生长培养基也会影响硫氢化物硫含量。还检测了来自小鼠和虾不同器官的总硫氢化物硫含量，范围为 1 至 10 nmol/（mg 蛋白质）。因此，新方法适用于生物样品中总硫氢化物硫的定量。
