Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang, China.
Kaohsiung J Med Sci. 2019 Dec;35(12):731-738. doi: 10.1002/kjm2.12109. Epub 2019 Aug 22.
The expression pattern, functions, and detailed regulatory mechanisms of miR-379 in laryngeal carcinoma remain unknown. In the present study, we aimed to uncover novel potential miR-379 targets and shed light on its roles in laryngeal carcinoma. The expression level of miR-379 was measured based on the data obtained from the TCGA database and the cell lines. After miR-379 mimic transfection, cell proliferation, invasion and migration assay, and wound-healing assay in HEp-2 cell line were implemented to evaluate the effects of miR-379 on laryngeal carcinoma in vitro. The target genes for miR-379 in silico were predicted and validated using luciferase reporter assay. The miR-379 expression level was reduced in laryngeal carcinoma tissues and cell lines. Moreover, miR-379 overexpression inhibited the cell proliferation, migration, and invasion of laryngeal carcinoma cells. TCF-4 was detected as a direct target of miR-379 in laryngeal carcinoma. Furthermore, restored TCF-4 expression rescued the inhibitory roles of miR-379 overexpression on cell proliferation, migration, and invasion.Our study for the first time demonstrated that miR-379/TCF-4 might involve in the progression of laryngeal carcinoma, and miR-379 appears to serve as a novel tumor suppressor in laryngeal carcinoma.
miR-379 在喉癌中的表达模式、功能及其详细的调控机制尚不清楚。本研究旨在揭示 miR-379 的新的潜在靶标，并阐明其在喉癌中的作用。根据 TCGA 数据库和细胞系获得的数据来测量 miR-379 的表达水平。在 HEp-2 细胞系中转染 miR-379 模拟物后，进行细胞增殖、侵袭和迁移实验以及划痕愈合实验，以评估 miR-379 对体外喉癌的影响。使用荧光素酶报告基因实验对 miR-379 的靶基因进行预测和验证。miR-379 在喉癌组织和细胞系中的表达水平降低。此外，miR-379 过表达抑制喉癌细胞的增殖、迁移和侵袭。在喉癌中，TCF-4 被检测为 miR-379 的直接靶基因。此外，恢复 TCF-4 的表达可挽救 miR-379 过表达对细胞增殖、迁移和侵袭的抑制作用。我们的研究首次表明，miR-379/TCF-4 可能参与喉癌的进展，miR-379 似乎在喉癌中作为一种新的肿瘤抑制因子。
