Garner R C, Tierney B, Phillips D H
Cancer Research Unit, University of York, Heslington, UK.
IARC Sci Publ. 1988(89):196-200.
Human lung DNA isolated from surgical specimens has been examined for the presence of polycyclic aromatic hydrocarbon-DNA adducts using both 32P-postlabelling and immunological methods. Of 12 samples examined to date, five had detectable amounts of benzo[a]pyrene diol epoxide-DNA adducts (BPDE-DNA) as determined by the enzyme-linked immunosorbent assay (ELISA), after immunoaffinity concentration. Values ranged from 3.5 to 11.5 fmol/mg DNA. When the same group of samples was analysed using the 32P-postlabelling technique, adducts could be detected in all the samples examined. There was generally not a good correspondence between the two methods. The number of adducts measured by 32P-postlabelling ranged from 1-100 per 10(8) nucleotides, which is some two orders of magnitude higher than with the immunological method, indicating that the BPDE-DNA adduct is probably not the major adduct present in these samples.
已使用³²P后标记法和免疫学法，对从手术标本中分离出的人类肺DNA进行了多环芳烃-DNA加合物检测。在迄今检测的12个样本中，经免疫亲和富集后，通过酶联免疫吸附测定法（ELISA）测定，有5个样本含有可检测量的苯并[a]芘二醇环氧化物-DNA加合物（BPDE-DNA）。含量范围为3.5至11.5 fmol/mg DNA。当使用³²P后标记技术分析同一组样本时，在所检测的所有样本中均可检测到加合物。这两种方法之间通常没有很好的对应关系。³²P后标记法测得的加合物数量为每10⁸个核苷酸1至100个，比免疫学法高出约两个数量级，这表明BPDE-DNA加合物可能不是这些样本中的主要加合物。
