Kriek E, Van Schooten F J, Hillebrand M J, Welling M C
Division of Chemical Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
IARC Sci Publ. 1988(89):201-7.
Antibodies raised against the bovine serum albumin conjugates of N-(guanosin-8-yl)-N-2-acetylaminofluorene (Guo-8-AAF), the imidazole ring-opened form of N-(guanosin-8-yl)-2-aminofluorene (roGuo-8-AF) and the methylated bovine serum albumin complex of DNA modified with (+/-)trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyre ne (BPDE) have been employed in a highly sensitive enzyme-linked immunosorbent assay (ELISA) to determine their affinity for DNA modified with the corresponding carcinogens at various levels of modification. All antibodies recognized highly modified DNA more efficiently than DNA of low modification. This property, which may be common to all antibodies raised against carcinogen-DNA adducts, has to be taken into account when these antibodies are used to quantify carcinogen-DNA adducts in biological samples. Appropriate DNA preparations of low modification have to be used as reference compounds in immunoassays to allow reliable quantification of adduct levels in DNA from animals and human cells.
针对N-（鸟苷-8-基）-N-2-乙酰氨基芴（Guo-8-AAF）、N-（鸟苷-8-基）-2-氨基芴的咪唑环开环形式（roGuo-8-AF）以及用（±）反式-7,8-二羟基-反式-9,10-环氧-7,8,9,10-四氢苯并[a]芘（BPDE）修饰的DNA的甲基化牛血清白蛋白复合物产生的抗体，已被用于一种高度灵敏的酶联免疫吸附测定（ELISA）中，以确定它们对用相应致癌物在不同修饰水平下修饰的DNA的亲和力。所有抗体识别高度修饰的DNA比识别低修饰的DNA更有效。当使用这些抗体来定量生物样品中的致癌物-DNA加合物时，必须考虑到这一特性，它可能是所有针对致癌物-DNA加合物产生的抗体所共有的。在免疫测定中必须使用适当的低修饰DNA制剂作为参考化合物，以便可靠地定量来自动物和人类细胞的DNA中的加合物水平。
