Alejandro R, Feldman E C, Shienvold F L, Mintz D H
Department of Medicine, School of Medicine, University of Miami, FL 33101.
J Am Vet Med Assoc. 1988 Nov 1;193(9):1050-5.
Histopathologic and immunocytochemical alterations in the pancreas of 18 dogs with spontaneous diabetes mellitus were evaluated. In 5 of 18 dogs (28%), extensive pancreatic damage appeared to be responsible for the development of diabetes mellitus. In the other 13 dogs, there was substantial reduction in the number of well-granulated beta cells, but the number of well-granulated alpha and delta cells was normal in 70% and 85% of the dogs, respectively. Also, insulitis lesions composed of infiltrating mononuclear cells, predominantly lymphocytes, were observed in 6 of 13 dogs (46%), but evidence of islet-directed humoral autoimmunity was not detected. Each pancreas had dense Ia (class II antigen) expression on ductal epithelia but not on islet endocrine cells. The importance of the insulitis lesions and class II antigen expression on ductal epithelial cells remains unclear. Of the 18 dogs, 8 received multidonor intrahepatic islet allografts. Allograft rejection was prevented by administration of cyclosporin. Five dogs were administered cyclosporin continuously. One dog with an allograft failed to sustain euglycemia at 231 days after transplantation, and one dog with fasting euglycemia died of pneumonia at 186 days after transplantation. In the other dogs, euglycemia was sustained for periods that ranged from 253 to 716 days.
对18只自发性糖尿病犬的胰腺进行了组织病理学和免疫细胞化学改变评估。18只犬中有5只（28%），广泛的胰腺损伤似乎是糖尿病发生的原因。在另外13只犬中，颗粒丰富的β细胞数量大幅减少，但颗粒丰富的α细胞和δ细胞数量分别在70%和85%的犬中正常。此外，在13只犬中有6只（46%）观察到由浸润的单核细胞（主要是淋巴细胞）组成的胰岛炎病变，但未检测到针对胰岛的体液自身免疫证据。每个胰腺的导管上皮有密集的Ia（II类抗原）表达，但胰岛内分泌细胞没有。胰岛炎病变和导管上皮细胞上II类抗原表达的重要性仍不清楚。18只犬中有8只接受了多供体肝内胰岛同种异体移植。通过给予环孢素预防了同种异体移植排斥反应。5只犬持续给予环孢素。1只接受同种异体移植的犬在移植后231天未能维持正常血糖，1只空腹血糖正常的犬在移植后186天死于肺炎。在其他犬中，正常血糖维持的时间为253至716天。
