Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
Gachon Institute of Pharmaceutical Science, Gachon University, Incheon, 21936, Republic of Korea; College of Pharmacy, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon, 21936, Republic of Korea.
Phytochemistry. 2019 Nov;167:112085. doi: 10.1016/j.phytochem.2019.112085. Epub 2019 Aug 19.
Six undescribed phenolic derivatives along with thirty two known compounds were isolated from the twigs of Betula schmidtii. The chemical structures were characterized through extensive spectroscopic analysis and chemical methods. All known compounds were first isolated in this plant. The anti-inflammatory effect of the isolates was tested by measuring nitric oxide production in lipopolysaccharide-activated BV-2 cells. Isotachioside, 4-allyl-2-hydrophenyl 1-O-β-D-apiosyl-(1 → 6)-β-D-glucopyranoside, genistein 5-O-β-D-glucoside, and prunetinoside showed a slight potency to lower the NO production against LPS-activated microglia with IC values of 23.9, 25.3, 28.8, and 34.0 μM, respectively.
从桦木科植物黑桦的嫩枝中分离得到 6 个未见文献报道的酚性衍生物和 32 个已知化合物。通过波谱数据分析和化学方法鉴定了它们的化学结构。所有已知化合物均首次从该植物中分离得到。采用 LPS 激活的 BV-2 细胞测定 NO 产生量的方法，评价了各化合物的抗炎活性。异槲皮苷、4-丙烯基-2-羟基苯基 1-O-β-D-芹糖基-(1→6)-β-D-吡喃葡萄糖苷、染料木素 5-O-β-D-葡萄糖苷和李苷显示出一定的抑制 LPS 激活的小胶质细胞产生 NO 的能力，其 IC50 值分别为 23.9、25.3、28.8 和 34.0 μM。
