Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Hazard Mater. 2020 Jan 5;381:121012. doi: 10.1016/j.jhazmat.2019.121012. Epub 2019 Aug 14.
SnO nanoparticles (NPs) are promising materials for electrochemical, catalytic, and biomedical applications due to their high photosensitivity, suitable stability characteristics, wide band gap energy potential, and low cost. Doping SnO NPs with metallic elements such as Ag has been used to improve their efficiency. Despite their commercial importance, the current literature lacks investigations to determine their toxic effects on plant systems. In this study, SnO and Ag/SnO NPs were synthesized using polymer pyrolysis method and characterized by means of XRD, TEM, SEM, EDX, and DLS techniques. Subsequently, the toxicity of the synthesized NPs on cell viability, cell proliferation, and a number of oxidative stress markers were measured in tobacco cell cultures. SnO and Ag/SnO NPs were found to be polygonal in shape with the size range of 10-30 nm. Both NPs induced cytotoxicity by reducing the cell viability and cell proliferation in a dose-dependent manner. Furthermore, the generation of HO, phenolics, flavonoids, and increased activities of superoxide dismutase (SOD) and peroxidase (POD) were observed. According to the results, Ag-doping played a key role in the induction of toxicity in tobacco cell cultures. The obtained results confirmed that SnO and Ag/SnO NPs induced cytotoxicity in tobacco cells through oxidative stress.
SnO 纳米粒子（NPs）由于其高灵敏度、合适的稳定性、宽能带隙能量潜力和低成本，在电化学、催化和生物医学应用方面具有广阔的前景。掺杂金属元素如 Ag 的 SnO NPs 已被用于提高其效率。尽管具有商业重要性，但目前的文献缺乏对其对植物系统毒性影响的研究。在本研究中，使用聚合物热解法合成了 SnO 和 Ag/SnO NPs，并通过 XRD、TEM、SEM、EDX 和 DLS 技术进行了表征。随后，在烟草细胞培养物中测量了合成的 NPs 对细胞活力、细胞增殖和一些氧化应激标志物的毒性。SnO 和 Ag/SnO NPs 呈多边形，尺寸范围为 10-30nm。两种 NPs 均通过降低细胞活力和细胞增殖来诱导细胞毒性，且呈剂量依赖性。此外，还观察到 HO、酚类、类黄酮的产生以及超氧化物歧化酶（SOD）和过氧化物酶（POD）活性的增加。根据结果，Ag 掺杂在烟草细胞培养物的毒性诱导中发挥了关键作用。所得结果证实，SnO 和 Ag/SnO NPs 通过氧化应激诱导烟草细胞产生细胞毒性。
