Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran.
Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran.
Bioelectrochemistry. 2019 Dec;130:107347. doi: 10.1016/j.bioelechem.2019.107347. Epub 2019 Aug 9.
A multifunctional nanocomposite theranostic system is constructed of manganese oxide (MnO) nanoparticles (NPs), as a tumor diagnostic agent, in conjunction with polyacrylic acid (PAA), as a pH-sensitive drug delivery agent, and methotrexate (MTX), as a model of targeting agent and anticancer drug. Physicochemical characteristics of the MnO@PAA/MTX system is studied in detail by several techniques, including X-ray and Auger photoelectron spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, and electrochemical methods. The system performance is studied based on (i) in-vitro MRI measurements to support efficiency of the MnO@PAA NPs as a diagnostic agent, (ii) drug release performance of the MnO@PAA/MTX NPs at pHs of 5.4 and 7.4 through in-vitro method to evaluate application of the NPs as pH-sensitive nanocarriers for MTX, and (iii) impedance spectroscopy measurements to show MnO@PAA/MTX NPs affinity for capturing of cancer cells. The results show that (i) MnO@PAA NPs can be used as a contrast agent in MRI measurements (r ≅ 6.5 mM s), (ii) the MTX, loaded on MnO@PAA NPs, is released faster and more efficient at pH 5.4 than 7.4, and (iii) the GC-MnO@PAA/MTX electrode system captures the 4T1 cells 3.32 times larger than L929 cells.
构建了一种多功能的纳米复合诊疗系统，由作为肿瘤诊断剂的氧化锰（MnO）纳米粒子（NPs）、作为 pH 敏感药物输送剂的聚丙烯酸（PAA）和作为靶向剂和抗癌药物模型的甲氨蝶呤（MTX）组成。采用 X 射线光电子能谱、俄歇光电子能谱、傅里叶变换红外光谱、X 射线衍射、场发射扫描电子显微镜、透射电子显微镜和电化学等多种技术详细研究了 MnO@PAA/MTX 体系的物理化学性质。该系统的性能是基于（i）体外 MRI 测量来支持 MnO@PAA NPs 作为诊断剂的效率，（ii）通过体外方法研究 MnO@PAA/MTX NPs 在 pH 5.4 和 7.4 下的药物释放性能，以评估 NPs 作为 MTX 的 pH 敏感纳米载体的应用，以及（iii）阻抗谱测量来显示 MnO@PAA/MTX NPs 对捕获癌细胞的亲和力。结果表明：（i）MnO@PAA NPs 可用作 MRI 测量中的造影剂（r≈6.5 mM s）；（ii）负载在 MnO@PAA NPs 上的 MTX 在 pH 5.4 下比 pH 7.4 下释放更快、更有效；（iii）GC-MnO@PAA/MTX 电极系统对 4T1 细胞的捕获量比 L929 细胞大 3.32 倍。
