Esparza I, González J C, Viñuela E
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Canto Blanco, Madrid, Spain.
J Gen Virol. 1988 Dec;69 ( Pt 12):2973-80. doi: 10.1099/0022-1317-69-12-2973.
Bovine interferon-alpha I1 (IFN-alpha I1) and porcine interferon-gamma (IFN-gamma) inhibited African swine fever virus replication in both porcine monocytes and alveolar macrophages. The most potent antiviral activity was observed with IFN-gamma-treated alveolar macrophages. The production of both a virulent (CC83) and a non-virulent (BA71) isolate of the virus was inhibited. Bovine tumour necrosis factor alpha did not show antiviral activity in either monocytes or alveolar macrophages. Rather, an increase of African swine fever virus production in tumour necrosis factor alpha-treated monocytes was found. An analysis of viral protein synthesis in IFN-alpha I1- and IFN-gamma-treated alveolar macrophages showed an inhibition of synthesis of some viral proteins. The inhibition of late proteins was very pronounced in IFN-gamma-treated cells, and it was probably a consequence of the inhibition of African swine fever virus DNA polymerase activity.
牛α-干扰素I1（IFN-α I1）和猪γ-干扰素（IFN-γ）在猪单核细胞和肺泡巨噬细胞中均能抑制非洲猪瘟病毒复制。在经IFN-γ处理的肺泡巨噬细胞中观察到最强的抗病毒活性。该病毒的强毒株（CC83）和无毒株（BA71）的产生均受到抑制。牛肿瘤坏死因子α在单核细胞或肺泡巨噬细胞中均未表现出抗病毒活性。相反，在经肿瘤坏死因子α处理的单核细胞中发现非洲猪瘟病毒产量增加。对经IFN-α I1和IFN-γ处理的肺泡巨噬细胞中病毒蛋白合成的分析表明，一些病毒蛋白的合成受到抑制。在经IFN-γ处理的细胞中，晚期蛋白的抑制非常明显，这可能是非洲猪瘟病毒DNA聚合酶活性受到抑制的结果。
