Paez E, Garcia F, Gil Fernandez C
Department of Virology, Centro de Investigaciones Biologicas (CSIC), Madrid, Spain.
Arch Virol. 1990;112(1-2):115-27. doi: 10.1007/BF01348989.
Human interferon alpha (IFN-alpha) and interferon gamma (IFN-gamma) inhibit African swine fever (ASF) virus replication in Vero cells. IFN-alpha and IFN-gamma exert a synergistic inhibition. Human tumor necrosis factor (TNF) does not inhibit ASF virus replication in this cell line, but in combination with IFNs it has antiviral enhancing activity. Analysis of the mechanism of inhibition suggests that the action of these cytokines blocks a step that comes prior to DNA replication. The 2'-5' A synthetase activity is induced in Vero cells by treatment with these cytokines and is activated after ASF virus infection. More interesting is the finding that continuous treatment with IFN-alpha cures Vero cells from lytic and persistent infections with ASF virus. A potential application of IFN for the treatment of animals carrying the virus is suggested.
人α干扰素（IFN-α）和γ干扰素（IFN-γ）可抑制非洲猪瘟（ASF）病毒在Vero细胞中的复制。IFN-α和IFN-γ发挥协同抑制作用。人肿瘤坏死因子（TNF）在该细胞系中不抑制ASF病毒复制，但与IFN联合使用时具有抗病毒增强活性。对抑制机制的分析表明，这些细胞因子的作用阻断了DNA复制之前的一个步骤。用这些细胞因子处理可在Vero细胞中诱导2'-5'A合成酶活性，且在ASF病毒感染后被激活。更有趣的是发现用IFN-α持续处理可使Vero细胞从ASF病毒的裂解性和持续性感染中治愈。这提示了IFN在治疗携带该病毒动物方面的潜在应用。
