Sen Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupinar University, Evliya Çelebi Campus, 43100, Kütahya, Turkey.
Department of Environmental Engineering, Faculty of Engineering, University of Igdir, Igdir, Turkey.
Sci Rep. 2019 Aug 22;9(1):12258. doi: 10.1038/s41598-019-48802-0.
The ultimate aim of this study is to produce a composite of bimetallic platinum-cobalt nanoparticles and reduced graphene oxide (Pt-Co@rGO) based biosensor for the detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Those are biologically important molecules with the key functions for the human body. Pt-Co@rGO was synthesized using a microwave-assisted technique and utilized for the production of a highly sensitive and stable electrochemical biosensor. Detailed spectral XPS and Raman analysis, XRD, and TEM/HR-TEM characterization were also studied. Due to the superior activity and excellent conductivity of rGO, well-separated oxidation peaks of these biomolecules is proven by DPV (differential pulse voltammetry) and CV (cyclic voltammetry) measurements. The prepared Pt-Co@rGO-based biosensor showed high electrochemical activity, a broad linear response, high sensitivity, and acceptable limit of detection values for individual and simultaneous determination of AA, DA, and UA, under optimized conditions. The linear range of Pt-Co@rGO was found to be 170-200; 35-1500 and 5-800 µM for AA, DA, and UA, respectively. Moreover, the detection limit of the prepared composite was calculated as 0.345; 0.051; 0.172 µM for AA, DA, and UA, respectively. In the field of electrochemical biosensors, Pt-Co@rGO based sensor is highly promising due to its superior sensitivity and good selectivity properties.
本研究的最终目的是制备一种基于双金属铂钴纳米粒子和还原氧化石墨烯（Pt-Co@rGO）的生物传感器，用于检测抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）。这些都是具有关键功能的生物重要分子。Pt-Co@rGO 是通过微波辅助技术合成的，并用于制备高灵敏度和稳定的电化学生物传感器。还对详细的光谱 XPS 和拉曼分析、XRD 和 TEM/HR-TEM 特性进行了研究。由于 rGO 的卓越活性和优异导电性，通过 DPV（差分脉冲伏安法）和 CV（循环伏安法）测量证明了这些生物分子的氧化峰得到了很好的分离。在优化条件下，制备的基于 Pt-Co@rGO 的生物传感器表现出高电化学活性、宽线性响应、高灵敏度和可接受的单个和同时测定 AA、DA 和 UA 的检测限值。Pt-Co@rGO 的线性范围分别为 170-200；35-1500 和 5-800 μM 用于 AA、DA 和 UA。此外，所制备的复合材料的检测限分别计算为 0.345；0.051；0.172 μM 用于 AA、DA 和 UA。在电化学生物传感器领域，基于 Pt-Co@rGO 的传感器因其卓越的灵敏度和良好的选择性而具有很高的应用前景。
