49.6-kDa亚单位菌毛蛋白抗原作为感染早期快速检测的潜在生物标志物。
Antigen of 49.6-kDa subunitpili protein of as a potential biomarker for early and rapid detection of the infection.
作者信息
Suharsono Hamong, Muttaqin Zainul, Tenaya I Wayan Masa, Agustina Kadek Karang, Prawiro Sumarno Retro
机构信息
Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
Biomedical Research Unit, West Nusa Tenggara General Hospital, Lombok, Indonesia.
出版信息
Vet World. 2019 Jun;12(6):769-773. doi: 10.14202/vetworld.2019.769-773. Epub 2019 Jun 10.
BACKGROUND AND AIM
infection has been identified as a major cause of peptic ulcer diseases, including gastric and duodenal ulcers, gastritis, chronic and gastric carcinoma, and even gastric lymphoma. studies using Western blotting analysis, hemagglutination test, adherence inhibition assays, and immunocytochemical staining revealed that the 49.6-kDa subunit pili protein of was considered an immunogenic protein. This study aimed to develop a serological diagnostic test using 49.6 kDa for detecting antibodies against proteins in an early phase of the infection.
MATERIALS AND METHODS
An in-house immunochromatographic test (ICT) kit was developed and used to test a panel of sera sample obtained from a randomly selected symptomatic patient, in which 40 sera were positive and 40 sera were negative.
RESULTS
The results showed that ICT with 49.6 kDa as an antigen was highly sensitive and specific for detecting anti- immunoglobulin G antibodies in human serum, with a high negative predictive value.
CONCLUSION
The developed test could be used to exclude infection in symptomatic patients.
背景与目的
感染已被确认为消化性溃疡疾病的主要病因，包括胃溃疡、十二指肠溃疡、胃炎、慢性和胃癌，甚至胃淋巴瘤。使用蛋白质印迹分析、血凝试验、黏附抑制试验和免疫细胞化学染色的研究表明，[细菌名称]的49.6 kDa亚基菌毛蛋白被认为是一种免疫原性蛋白。本研究旨在开发一种使用49.6 kDa的血清学诊断试验，用于在感染早期检测针对[细菌名称]蛋白的抗体。
材料与方法
开发了一种内部免疫层析试验（ICT）试剂盒，并用于检测从随机选择的有症状患者中获得的一组血清样本，其中40份血清为[细菌名称]阳性，40份血清为[细菌名称]阴性。
结果
结果表明，以49.6 kDa作为抗原的ICT对检测人血清中的抗[细菌名称]免疫球蛋白G抗体具有高度敏感性和特异性，阴性预测值高。
结论
所开发的试验可用于排除有症状患者的[细菌名称]感染。
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