Li Huawei, Yang Jifei, Bao Dengke, Hou Jie, Zhi Yubao, Yang Yanyan, Ji Pengchao, Zhou Enmin, Qiao Songlin, Zhang Gaiping
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
College of Biology Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China.
J Vet Sci. 2017 Sep 30;18(3):307-316. doi: 10.4142/jvs.2017.18.3.307.
A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.
一种简单快速的免疫层析试纸条成功研制出来，该试纸条包含胶体金标记的重组Nsp7抗原探针，用于检测猪血清中抗猪繁殖与呼吸综合征病毒（PRRSV）抗体。将胶体金标记的PRRSV重组Nsp7蛋白滴加在结合垫上作为检测物。将葡萄球菌蛋白A和纯化的猪抗Nsp7抗体分别点样在硝酸纤维素膜上形成检测线和对照线。还将该试纸条与标准诊断试验、酶联免疫吸附试验和免疫过氧化物酶单层试验进行了比较。结果表明，该免疫层析试纸条具有高特异性和高灵敏度。此外，该试纸条检测快速、操作简便，无需专业技能和设备。建议该免疫层析试纸条可用于快速准确地检测PRRSV抗体，适用于现场诊断。
