Department of Medical Biochemistry, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Gynecological Clinic 1, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Pregnancy Hypertens. 2019 Oct;18:1-8. doi: 10.1016/j.preghy.2019.08.006. Epub 2019 Aug 16.
Changes in the renin-angiotensin-aldosterone system's (RAAS) activity due to different genetic variations could represent risk factors for the onset of preeclampsia.
To test and quantify the relationships of 8 RAAS gene polymorphisms (angiotensinogen (AGT)-M235T, AGT-T174M, angiotensin converting enzyme (ACE)-I/D, ACE8-A2350G, angiotensin II type 1 receptor (AGTR1)-A1166C, angiotensin II type 2 receptor (AGTR2)-C3123A, renin (REN)-G83A, aldosterone synthase (CYP11B2)-T344C) with susceptibility to early- (EOPE) and late-onset preeclampsia (LOPE).
We performed polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) analysis in 217 pregnant women, of whom 87 pregnant women with EOPE/LOPE and 130 normal pregnant women. The relationship between the studied RASS gene polymorphisms and EOPE/LOPE was tested by multiple logistic regressions.
The multivariate logistic regression analysis showed that AGT-M235T (adjusted OR = 4.63), AGT-T174M (adjusted OR = 4.13), REN-G83A (adjusted OR = 3) and CYP11B2-C344T (adjusted OR = 3.13) gene polymorphisms remained independent risk factors for EOPE. Moreover, ACE-I/D (adjusted OR = 4.04), ACE-A2350G (adjusted OR = 3.5), AGTR1-A1166C (adjusted OR = 2.73), and REN-G83A (adjusted OR = 2.67) polymorphisms remained independent risk factors for LOPE. The frequency of overweight was significantly different (p = 0.001) in pregnant women with EOPE, LOPE and the control group (LOPE:16, 29.6% vs. EOPE:12, 36.4% vs. control group:16, 12.3%). Pregnant women with EOPE had babies with a significantly lower mean birth weight (2067.9 ± 887.9) in comparison to women with LOPE (mean ± SD: 2860.1 ± 771.1, p < 0.001) and women with normal pregnancies, respectively (mean ± SD: 3324.9 ± 484.9, p < 0.001).
We confirmed the influence of the renin-angiotensin-aldosterone system through these 8 genetic variations on the onset of preeclampsia.
由于不同的遗传变异，肾素-血管紧张素-醛固酮系统（RAAS）活性的变化可能是子痫前期发病的危险因素。
检测和量化 8 个 RAAS 基因多态性（血管紧张素原（AGT）-M235T、AGT-T174M、血管紧张素转换酶（ACE）-I/D、ACE8-A2350G、血管紧张素 II 型 1 型受体（AGTR1）-A1166C、血管紧张素 II 型 2 型受体（AGTR2）-C3123A、肾素（REN）-G83A、醛固酮合酶（CYP11B2）-T344C）与早发性（EOPE）和晚发性子痫前期（LOPE）易感性的关系。
我们对 217 名孕妇进行了聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析，其中 87 名患有 EOPE/LOPE 的孕妇和 130 名正常孕妇。通过多元逻辑回归分析研究的 RAAS 基因多态性与 EOPE/LOPE 的关系。
多变量逻辑回归分析显示，AGT-M235T（调整后的 OR=4.63）、AGT-T174M（调整后的 OR=4.13）、REN-G83A（调整后的 OR=3）和 CYP11B2-C344T（调整后的 OR=3.13）基因多态性仍然是 EOPE 的独立危险因素。此外，ACE-I/D（调整后的 OR=4.04）、ACE-A2350G（调整后的 OR=3.5）、AGTR1-A1166C（调整后的 OR=2.73）和 REN-G83A（调整后的 OR=2.67）多态性仍然是 LOPE 的独立危险因素。患有 EOPE、LOPE 和对照组的孕妇超重的频率有显著差异（p=0.001）（LOPE：16，29.6% vs. EOPE：12，36.4% vs. 对照组：16，12.3%）。与 LOPE 组（平均值±SD：2860.1±771.1，p<0.001）和正常妊娠组（平均值±SD：3324.9±484.9，p<0.001）相比，患有 EOPE 的孕妇的婴儿出生体重明显较低（2067.9±887.9）。
我们证实了肾素-血管紧张素-醛固酮系统通过这 8 个遗传变异对子痫前期发病的影响。
