Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea.
Food Chem. 2020 Jan 15;303:125376. doi: 10.1016/j.foodchem.2019.125376. Epub 2019 Aug 19.
This study investigated the effects of persistent ultraviolet B (UV-B) irradiation on isoflavone accumulation in soybean sprouts. Three malonyl isoflavones were increased by UV-B. Malonylgenistin specifically accumulated upon UV-B exposure, whereas the other isoflavones were significantly increased under both dark conditions and UV-B exposure. The results of isoflavone accumulation to UV-B irradiation time were observed as following: acetyl glycitin rapidly increased and then gradually decreased; malonyl daidzin and malonyl genistin were highly accumulated within an intermediate period; genistein and daidzin were gradually maximized; daidzin, glycitin, genistein, and malonyl glycitin did not increase; and glycitin, acetyl daidzin, and acetyl genistin exhibited trace amounts. Transcriptional analysis of isoflavonoid biosynthetic genes demonstrated that most metabolic genes were highly activated in response to UV-B 24 and UV-B 36 treatments. In particular, it was found that GmCHS6, GmCHS7, and GmCHS8 genes among the eight known genes encoding chalcone synthase were specifically related to UV-B response.
本研究探讨了持续的紫外线 B（UV-B）照射对大豆芽中异黄酮积累的影响。三种丙二酰基异黄酮增加了 UV-B。丙二酰基染料木黄酮在 UV-B 暴露下特异性积累，而其他异黄酮在黑暗条件和 UV-B 暴露下均显著增加。异黄酮积累对 UV-B 照射时间的结果如下：乙酰基甘草苷迅速增加，然后逐渐减少；丙二酰基大豆苷和丙二酰基染料木黄酮在中期高度积累；染料木黄酮和大豆苷逐渐达到最大值；大豆苷、甘草苷、染料木黄酮和丙二酰基甘草苷没有增加；而甘草苷、乙酰基大豆苷和乙酰基染料木黄酮则微量存在。异黄酮生物合成基因的转录分析表明，大多数代谢基因在 UV-B 24 和 UV-B 36 处理下高度激活。特别是，发现八个已知编码查尔酮合酶的基因中的 GmCHS6、GmCHS7 和 GmCHS8 基因与 UV-B 反应特别相关。
