缺氧迅速诱导人脂肪来源的贴壁基质细胞中的心源性因子表达。

Hypoxia Rapidly Induces the Expression of Cardiomyogenic Factors in Human Adipose-Derived Adherent Stromal Cells.

作者信息

Choi Jung-Won, Moon Hanbyeol, Jung Seung Eun, Lim Soyeon, Lee Seahyoung, Kim Il-Kwon, Lee Hoon-Bum, Lee Jiyun, Song Byeong-Wook, Kim Sang Woo, Hwang Ki-Chul

机构信息

Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 210-701, Korea.

Department of Integrated Omics for Biomedical Sciences, Graduate School, Yonsei University, Seoul 03722, Korea.

出版信息

J Clin Med. 2019 Aug 15;8(8):1231. doi: 10.3390/jcm8081231.

DOI:10.3390/jcm8081231

PMID:31443313

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6723458/

Abstract

BACKGROUND

The efficacy of interstitial vascular fraction (SVF) transplantation in the treatment of heart disease has been proven in a variety of in vivo studies. In a previous study, we found that bone marrow-derived mesenchymal stem cells (BM-MSCs) altered their expression of several cardiomyogenic factors under hypoxic conditions.

METHODS

We hypothesized that hypoxia may also induce obtained adipose-derived adherent stromal cells (ADASs) from SVFs and adipose-derived stem cells (ASCs) to differentiate into cardiomyocytes and/or cells with comparable phenotypes. We examined the differentiation markers of cell lineages in ADASs and ASCs according to time by hypoxic stress and found that only ADASs expressed cardiomyogenic markers within 24 h under hypoxic conditions in association with the expression of hypoxia-inducible factor 1-α (HIF-1α).

RESULTS

Differentially secreted proteins in a conditioned medium (CM) from ASCs and ADASs under normoxic or hypoxic conditions were detected using an antibody assay and may be associated with a dramatic increase in the expression of cardiomyogenic markers in only ADASs. Furthermore, the cardiomyogenic factors were expressed more rapidly in ADASs than in ASCs under hypoxic conditions in association with the expression of HIF-1α, and angiogenin, fibroblast growth factor-19 (FGF-19) and/or macrophage inhibitory factor (MIF) are related.

CONCLUSIONS

These results provide new insights into the applicability of ADASs preconditioned by hypoxic stress in cardiac diseases.

摘要

背景

间质血管组分（SVF）移植治疗心脏病的疗效已在多种体内研究中得到证实。在先前的一项研究中，我们发现骨髓间充质干细胞（BM-MSCs）在缺氧条件下会改变其几种心肌生成因子的表达。

方法

我们假设缺氧也可能诱导从SVF和脂肪来源干细胞（ASC）获得的脂肪来源贴壁基质细胞（ADAS）分化为心肌细胞和/或具有类似表型的细胞。我们通过缺氧应激按时间检查了ADAS和ASC中细胞谱系的分化标志物，发现只有ADAS在缺氧条件下24小时内表达心肌生成标志物，并伴有缺氧诱导因子1-α（HIF-1α）的表达。

结果

使用抗体检测法检测了常氧或缺氧条件下ASC和ADAS条件培养基（CM）中差异分泌的蛋白质，这些蛋白质可能与仅ADAS中心肌生成标志物表达的显著增加有关。此外，在缺氧条件下，与HIF-1α的表达相关，ADAS中心肌生成因子的表达比ASC更快，且血管生成素、成纤维细胞生长因子-19（FGF-19）和/或巨噬细胞抑制因子（MIF）与之相关。