Lim Soyeon, Kim Il-Kwon, Choi Jung-Won, Seo Hyang-Hee, Lim Kyu Hee, Lee Seahyoung, Lee Hoon-Bum, Kim Sang Woo, Hwang Ki-Chul
Catholic Kwandong University, International St. Mary's Hospital, Incheon Metropolitan City, 22711, Republic of Korea.
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do 25601, Republic of Korea.
Int J Med Sci. 2017 Jul 20;14(9):911-919. doi: 10.7150/ijms.19998. eCollection 2017.
Stromal vascular fractions (SVFs) are a heterogeneous collection of cells within adipose tissue that are being studied for various clinical indications. In this study, we aimed to determine whether SVF transplantation into impaired tissues has differential effects on inflammatory and angiogenetic properties with regard to gender. As reactive oxygen species have been implicated in cardiovascular disease development, we investigated differences in gene and protein expression related to inflammation and angiogenesis in HUVECs co-cultured with adipose-derived SVFs from male (M group) and female (F group) individuals under oxidative stress conditions. The expression of several inflammatory (interleukin (IL)-33) and angiogenetic (platelet-derived growth factor (PDGF)) factors differed dramatically between male and female donors. Anti-inflammatory and pro-angiogenetic responses were observed in HUVECs co-cultured with SVFs under oxidative stress conditions, and these characteristics may exhibit partially differential effects according to gender. Using network analysis, we showed that co-culturing HUVECs with SVFs ameliorated pyroptosis/apoptosis via an increase in oxidative stress. Activation of caspase-1 and IL-1B was significantly altered in HUVECs co-cultured with SVFs from female donors. These findings regarding gender-dimorphic regulation of adipose-derived SVFs provide valuable information that can be used for evidence-based gender-specific clinical treatment of SVF transplantation for understanding of cardiovascular disease, allowing for the development of additional treatment.
基质血管组分（SVFs）是脂肪组织内的一组异质性细胞，目前正针对各种临床适应症进行研究。在本研究中，我们旨在确定将SVF移植到受损组织中对炎症和血管生成特性是否具有性别差异效应。由于活性氧与心血管疾病的发展有关，我们研究了在氧化应激条件下，与来自男性（M组）和女性（F组）个体的脂肪源性SVF共培养的人脐静脉内皮细胞（HUVECs）中与炎症和血管生成相关的基因和蛋白表达差异。几种炎症因子（白细胞介素（IL）-33）和血管生成因子（血小板衍生生长因子（PDGF））的表达在男性和女性供体之间存在显著差异。在氧化应激条件下，与SVF共培养的HUVECs中观察到抗炎和促血管生成反应，并且这些特征可能根据性别表现出部分差异效应。通过网络分析，我们表明将HUVECs与SVF共培养可通过增加氧化应激来改善细胞焦亡/凋亡。与来自女性供体的SVF共培养的HUVECs中，半胱天冬酶-1和IL-1B的激活发生了显著改变。这些关于脂肪源性SVF性别二态性调节的发现提供了有价值的信息，可用于基于证据的性别特异性SVF移植临床治疗，以了解心血管疾病，从而开发更多治疗方法。
