National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
Grain Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Int J Mol Sci. 2019 Aug 22;20(17):4103. doi: 10.3390/ijms20174103.
The basic leucine zipper () family of transcription factors (TFs) regulate diverse phenomena during plant growth and development and are involved in stress responses and hormone signaling. However, only a few have been functionally characterized. In this paper, 54 maize genes were screened from previously published drought and rewatering transcriptomes. These genes were divided into nine groups in a phylogenetic analysis, supported by motif and intron/exon analyses. The 54 genes were unevenly distributed on 10 chromosomes and contained 18 segmental duplications, suggesting that segmental duplication events have contributed to the expansion of the maize family. Spatio-temporal expression analyses showed that genes are widely expressed during maize development. We identified 10 core involved in protein transport, transcriptional regulation, and cellular metabolism by principal component analysis, gene co-expression network analysis, and Gene Ontology enrichment analysis. In addition, 15 potential stress-responsive ZmbZIPs were identified by expression analyses. Localization analyses showed that , , , and are nuclear proteins. These results provide the basis for future functional genomic studies on TFs in maize and identify candidate genes with potential applications in breeding/genetic engineering for increased stress resistance. These data represent a high-quality molecular resource for selecting resistant breeding materials.
基本亮氨酸拉链（bZIP）转录因子（TFs）家族调节植物生长发育过程中的多种现象，并参与应激反应和激素信号转导。然而，只有少数 bZIP 已被功能表征。本文从先前发表的干旱和复水转录组中筛选出 54 个玉米 bZIP 基因。这些基因在系统发育分析中分为九个组， motif 和内含子/外显子分析提供了支持。这 54 个基因不均匀分布在 10 条染色体上，包含 18 个片段重复，表明片段重复事件有助于玉米 bZIP 家族的扩张。时空表达分析表明，在玉米发育过程中， 基因广泛表达。通过主成分分析、基因共表达网络分析和基因本体论富集分析，我们鉴定了 10 个核心 bZIP 参与蛋白质运输、转录调控和细胞代谢。此外，通过表达分析鉴定了 15 个潜在的应激响应 ZmbZIPs。定位分析表明， 、 、 、 和 是核蛋白。这些结果为未来玉米 bZIPs 功能基因组研究提供了基础，并鉴定了具有增加抗逆性的潜在应用的候选基因。这些数据为选择抗逆性育种材料提供了高质量的分子资源。
