血红素•G-四链体脱氧核酶：使其过氧化物酶活性最大化的条件

Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity.

作者信息

Shumayrikh Nisreen, Sen Dipankar

机构信息

Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada.

Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.

出版信息

Methods Mol Biol. 2019;2035:357-368. doi: 10.1007/978-1-4939-9666-7_22.

DOI:10.1007/978-1-4939-9666-7_22

PMID:31444762

Abstract

Catalytic DNAs (DNAzymes) with peroxidase-like activity have great potential in bioanalytical chemistry [1], owing to numerous advantages that DNA enzymes offer over conventional protein enzymes, including structural simplicity, low cost, thermal stability, and straightforward handling and preparation. Maximizing the efficiency of the peroxidase activity of such DNAzymes is a subject in need of review. In this chapter, we discuss the optimal experimental conditions for the peroxidase activity of these DNAzymes and describe general procedures for their utilization.

摘要

具有过氧化物酶样活性的催化性DNA（DNA酶）在生物分析化学领域具有巨大潜力[1]，这归因于DNA酶相对于传统蛋白质酶所具有的众多优势，包括结构简单、成本低、热稳定性好以及操作和制备简便。最大化此类DNA酶的过氧化物酶活性效率是一个亟待综述的课题。在本章中，我们将讨论这些DNA酶过氧化物酶活性的最佳实验条件，并描述其应用的一般程序。